ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1999
Differentiation of mammalian vestibular hair cells from conditionally immortal, postnatal supporting cells.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 49 citations in OpenAlex.
- Transcription factor STOX1 regulates proliferation of inner ear epithelial cells via the AKT pathway.Cell proliferation · 2015Article
- Plastin family of actin-bundling proteins: its functions in leukocytes, neurons, intestines, and cancer.International journal of cell biology · 2012Article
- Role of protein misfolding in DFNA9 hearing loss.The Journal of biological chemistry · 2010Article
- Emx2 and early hair cell development in the mouse inner ear.Developmental biology · 2010Article
- Stem/progenitor cells derived from the cochlear sensory epithelium give rise to spheres with distinct morphologies and features.Journal of the Association for Research in Otolaryngology : JARO · 2009Article
- Using the zebrafish lateral line to screen for ototoxicity.Journal of the Association for Research in Otolaryngology : JARO · 2008Article
- Development and regeneration of hair cells share common functional features.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Inner ear hair cells produced in vitro by a mesenchymal-to-epithelial transition.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Developmental acquisition of voltage-dependent conductances and sensory signaling in hair cells of the embryonic mouse inner ear.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Myo6 facilitates the translocation of endocytic vesicles from cell peripheries.Molecular biology of the cell · 2003Article
- Transcript profiling of functionally related groups of genes during conditional differentiation of a mammalian cochlear hair cell line.Genome research · 2002Article
- The adenomatous polyposis coli protein unambiguously localizes to microtubule plus ends and is involved in establishing parallel arrays of microtubule bundles in highly polarized epithelial cells.The Journal of cell biology · 2002Article
- Cellular studies of auditory hair cell regeneration in birds.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We provide evidence from a newly established, conditionally immortal cell line (UB/UE-1) that vestibular supporting cells from the mammalian inner ear can differentiate postnatally into more than one variant of hair cell. A clonal supporting cell line was established from pure utricular sensory epithelia of H2k(b)tsA58 transgenic mice 2 d after birth. Cell proliferation was dependent on conditional expression of the immortalizing gene, the "T" antigen from the SV40 virus. Proliferating cells expressed cytokeratins, and patch-clamp recordings revealed that they all expressed small membrane currents with little time-dependence. They stopped dividing within 2 d of being transferred to differentiating conditions, and within a week they formed three defined populations expressing membrane currents characteristic of supporting cells and two kinds of neonatal hair cell. The cells expressed several characteristic features of normal hair cells, including the transcription factor Brn3.1, a functional acetylcholine receptor composed of alpha9 subunits, and the cytoskeletal proteins myosin VI, myosin VIIa, and fimbrin. Immunofluorescence labeling and electron microscopy showed that the cells formed complex cytoskeletal arrays on their upper surfaces with structural features resembling those at the apices of normal hair cells. The cell line UB/UE-1 provides a valuable in vitro preparation in which the expression of numerous structural and physiological components can be initiated or upregulated during early stages of mammalian hair cell commitment and differentiation.
Indexed as
Identifiers
10531448PMC6782912W2116690187What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.