ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1999
Excitatory synaptogenesis between identified Lymnaea neurons requires extrinsic trophic factors and is mediated by receptor tyrosine kinases.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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Who cites it
29 citing papers in PubMed, 79 citations in OpenAlex.
- Neuronal growth patterns and synapse formation are mediated by distinct activity-dependent mechanisms.Scientific reports · 2025Article
- Dopamine-mediated calcium channel regulation in synaptic suppression in L. stagnalis interneurons.Channels (Austin, Tex.) · 2018Article
- The role of retinoic acid in the formation and modulation of invertebrate central synapses.Journal of neurophysiology · 2017Article
- Trophic factor-induced activity 'signature' regulates the functional expression of postsynaptic excitatory acetylcholine receptors required for synaptogenesis.Scientific reports · 2015Article
- In vitro studies of neuronal networks and synaptic plasticity in invertebrates and in mammals using multielectrode arrays.Neural plasticity · 2015Review
- Retinoic acid affects calcium signaling in adult molluscan neurons.Journal of neurophysiology · 2015Article
- Neuronal somata and extrasomal compartments play distinct roles during synapse formation between Lymnaea neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Retinoic acid induces changes in electrical properties of adult neurons in a dose- and isomer-dependent manner.Journal of neurophysiology · 2014Article
- Article
- Molluscan neurons in culture: shedding light on synapse formation and plasticity.Journal of molecular histology · 2012Review
- Lidocaine treatment during synapse reformation periods permanently inhibits NGF-induced excitation in an identified reconstructed synapse of Lymnaea stagnalis.Journal of anesthesia · 2012Article
- Regulation and restoration of motoneuronal synaptic transmission during neuromuscular regeneration in the pulmonate snail Helisoma trivolvis.The Biological bulletin · 2011Article
- From understanding cellular function to novel drug discovery: the role of planar patch-clamp array chip technology.Frontiers in pharmacology · 2011Article
- Quercetin targets cysteine string protein (CSPalpha) and impairs synaptic transmission.PloS one · 2010Article
- A novel, nongenomic mechanism underlies retinoic acid-induced growth cone turning.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Trophic factor-induced intracellular calcium oscillations are required for the expression of postsynaptic acetylcholine receptors during synapse formation between Lymnaea neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- The role of synaptotagmin I C2A calcium-binding domain in synaptic vesicle clustering during synapse formation.The Journal of physiology · 2007Article
- Activation of epidermal growth factor receptor mediates receptor axon sorting and extension in the developing olfactory system of the moth Manduca sexta.The Journal of comparative neurology · 2006Article
- Protein synthesis-dependent reactivation of a contextual conditioned reflex in the common snail.Neuroscience and behavioral physiology · 2006Article
- Inhibition of neurotransmitter release by a nonphysiological target requires protein synthesis and involves cAMP-dependent and mitogen-activated protein kinases.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neurotrophic factors have well established roles in neuronal development and adult synaptic plasticity, but their precise role in synapse formation has yet to be determined. This paper provides the first direct evidence that neurotrophic factors in brain conditioned medium (CM) differentially regulate excitatory and inhibitory synapse formation. Somata of identified presynaptic and postsynaptic neurons were isolated from the CNS of Lymnaea and were cultured in a soma-soma configuration in the presence (CM) or absence [defined medium (DM)] of trophic factors. In DM, excitatory synapses did not form. When they were paired in CM or in DM containing Lymnaea epidermal growth factor (EGF); however, all presynaptic neurons reestablished their specific excitatory synapses, which had electrical properties similar to those seen in vivo. CM-induced formation of excitatory synapses required transcription and de novo protein synthesis, as indicated by the observations that synapse formation was blocked by the protein synthesis inhibitor anisomycin and the protein transcription blocker actinomycin D; the CM factor was inactivated by boiling. They were also blocked by receptor tyrosine kinase inhibitors (lavendustin A, genistein, K252a, and KT5926) but not by inactive analogs (genistin and lavendustin B), suggesting that the effect was mediated by receptor tyrosine kinases. These results, together with our previously published data, demonstrate that trophic factors are required for excitatory, but not inhibitory, synapse formation and extends the role of EGF from cell proliferation, neurite outgrowth, and survival to excitatory synapse formation.
Indexed as
Identifiers
10531435PMC6782902W1688827157What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.