ArticleThe Biochemical journal1999
Glycosylphosphatidylinositol-anchor intermediates associate with triton-insoluble membranes in subcellular compartments that include the endoplasmic reticulum.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Cytochrome P450 Organization and Function Are Modulated by Endoplasmic Reticulum Phospholipid Heterogeneity.Drug metabolism and disposition: the biological fate of chemicals · 2016Review
- Role of the lipid rafts in the life cycle of canine coronavirus.The Journal of general virology · 2015Article
- Relationship between CYP1A2 localization and lipid microdomain formation as a function of lipid composition.Drug metabolism and disposition: the biological fate of chemicals · 2013Article
- Article
- Gas1 interferes with AβPP trafficking by facilitating the accumulation of immature AβPP in endoplasmic reticulum-associated raft subdomains.Journal of Alzheimer's disease : JAD · 2012Article
- CDP-diacylglycerol phospholipid synthesis in detergent-soluble, non-raft, membrane microdomains of the endoplasmic reticulum.Journal of lipid research · 2011Article
- Growth arrest-specific 1 binds to and controls the maturation and processing of the amyloid-beta precursor protein.Human molecular genetics · 2011Article
- Importance of cholesterol for infection of cells by transmissible gastroenteritis virus.Virus research · 2008Article
- Insights into the role of specific lipids in the formation and delivery of lipid microdomains to the plasma membrane of plant cells.Plant physiology · 2007Article
- Dissecting rotavirus particle-raft interaction with small interfering RNAs: insights into rotavirus transit through the secretory pathway.Journal of virology · 2006Article
- The association of Shiga-like toxin with detergent-resistant membranes is modulated by glucosylceramide and is an essential requirement in the endoplasmic reticulum for a cytotoxic effect.Molecular biology of the cell · 2006Article
- PrP(C) association with lipid rafts in the early secretory pathway stabilizes its cellular conformation.Molecular biology of the cell · 2004Article
- Effect of glycosylphosphatidylinositol (GPI)-phospholipase D overexpression on GPI metabolism.The Biochemical journal · 2004Article
- Ganglioside glycosyltransferases and newly synthesized gangliosides are excluded from detergent-insoluble complexes of Golgi membranes.The Biochemical journal · 2004Article
- Metabolism of exogenous sn-1-alkyl-sn-2-lyso-glucosaminyl-phosphatidylinositol in HeLa D cells: accumulation of glucosaminyl(acyl)phosphatidylinositol in a metabolically inert compartment.The Biochemical journal · 2001Article
- Early increase in DcR2 expression and late activation of caspases in the platelet storage lesion.Leukemia · 2001Article
- Sphingomyelin-enriched microdomains at the Golgi complex.Molecular biology of the cell · 2001Article
- Dissecting the role of the golgi complex and lipid rafts in biosynthetic transport of cholesterol to the cell surface.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Glycosylphosphatidylinositol (GPI)-anchored proteins are resistant to solubilization with Triton X-100 at 4 degrees C, and they can be recovered in Triton-insoluble membranes (TIMs) that float to a characteristic buoyant density. Because the GPI structure itself has been shown to target GPI-anchored proteins to TIMs, we investigated the association of GPI-anchor intermediates with TIMs. GPI-anchor biosynthesis involves a pathway of some 10 steps that take place in the endoplasmic reticulum (ER). These intermediates include glucosaminyl-acylphosphatidylinositol [GlcN-(acyl)PI] and later mannosylated GPIs, denoted H6, H7 and H8, that are present not only in the ER but also in other cell compartments, including the plasma membrane. At least two-thirds of the GlcN-(acyl)PI in HeLa D cells and mannosylated GPIs in K562 cells were found in TIMs. Although previous reports have considered TIMs to be derived primarily from the plasma membrane, we recovered TIMs from subcellular fractions enriched in ER membranes. The ER marker calnexin and GPI-anchored proteins as well as N-acetylglucosaminyl-phosphatidylinositol and mannosylated GPIs were present in ER-TIMs. Interestingly, GlcN-PI and H7 were less enriched in ER-TIM than the other GPIs, suggesting that ER-TIMs might reflect a compartmentalization of the GPI-anchor biosynthetic pathway in the ER.
Indexed as
Identifiers
10527942PMC1220595W2025155199What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.