ArticlePlant molecular biology1999
Semi-quantitative RT-PCR analysis of photoregulated gene expression in marine diatoms.
Article in Plant molecular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 64 citations in OpenAlex.
- Pelagic diatoms communicate through synchronized beacon natural fluorescence signaling.Science advances · 2021Article
- Diatom Phytochromes Reveal the Existence of Far-Red-Light-Based Sensing in the Ocean.The Plant cell · 2016Article
- Regulation of Intertidal Microphytobenthos Photosynthesis Over a Diel Emersion Period Is Strongly Affected by Diatom Migration Patterns.Frontiers in microbiology · 2016Article
- Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum.Scientific reports · 2015Article
- Comparison of oligomeric states and polypeptide compositions of fucoxanthin chlorophyll a/c-binding protein complexes among various diatom species.Photosynthesis research · 2013Article
- Genome-wide diel growth state transitions in the diatom Thalassiosira pseudonana.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Article
- Quantification of diatom gene expression in the sea by selecting uniformly transcribed mRNA as the basis for normalization.Applied and environmental microbiology · 2012Article
- Physiological and transcriptomic evidence for a close coupling between chloroplast ontogeny and cell cycle progression in the pennate diatom Seminavis robusta.Plant physiology · 2008Article
- Evolutionary origins and functions of the carotenoid biosynthetic pathway in marine diatoms.PloS one · 2008Article
- A bacteriophytochrome regulates the synthesis of LH4 complexes in Rhodopseudomonas palustris.Photosynthesis research · 2005Article
- Ectopic expression of OsYAB1 causes extra stamens and carpels in rice.Plant molecular biology · 2004Article
- The OsFOR1 gene encodes a polygalacturonase-inhibiting protein (PGIP) that regulates floral organ number in rice.Plant molecular biology · 2003Article
- Rapid evolution of a sexual reproduction gene in centric diatoms of the genus Thalassiosira.Applied and environmental microbiology · 2001Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The low cell densities of diatoms and other phytoplankton in culture has precluded the use of classical RNA analysis techniques for routine studies of gene expression in large numbers of samples. This has seriously hampered studies of the basic biology of such organisms. To circumvent this problem, we have developed a high-throughput semi-quantitative RT-PCR-based protocol and used it to monitor expression of a gene encoding a fucoxanthin, chlorophyll a/c-binding protein (FCP) in the centric planktonic diatom Thalassiosira weissflogii. Analysis of FCP gene expression in dark-adapted diatom cultures revealed that mRNA levels increase 5- to 6-fold in response to white light irradiation and peak around 6 to 8 h. To determine the photoreceptors involved in this response action spectra of FCP gene expression were determined using the Okazaki large spectrograph. Responses consistent with the presence of cryptochrome-, rhodopsin- and phytochrome-type receptors could be detected. The apparent presence of phytochrome-mediated responses is of particular interest given the low fluences of red and far-red light wavelengths in the marine environment.
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