ArticlePlant molecular biology1999
Construction and characterization of a common bean bacterial artificial chromosome library.
Article in Plant molecular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 38 citations in OpenAlex.
- The Co-4 locus on chromosome Pv08 contains a unique cluster of 18 COK-4 genes and is regulated by immune response in common bean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2015Article
- Bacterial artificial chromosome libraries of pulse crops: characteristics and applications.Journal of biomedicine & biotechnology · 2012Review
- Construction of a BAC library and a physical map of a major QTL for CBB resistance of common bean (Phaseolus vulgaris L.).Genetica · 2010Article
- Cytogenetic map of common bean (Phaseolus vulgaris L.).Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Novel bacterial artificial chromosome vector pUvBBAC for use in studies of the functional genomics of Listeria spp.Applied and environmental microbiology · 2008Article
- BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut.BMC plant biology · 2008Article
- Mitochondrial genome dynamics in plants and animals: convergent gene fusions of a MutS homologue.Journal of molecular evolution · 2006Article
- Development of four phylogenetically-arrayed BAC libraries and sequence of the APA locus in Phaseolus vulgaris.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
- Article
- The bacterial artificial chromosome (BAC) library of the narrow-leafed lupin (Lupinus angustifolius L.).Cellular & molecular biology letters · 2006Article
- Construction and characterization of a peanut HindIII BAC library.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- The anthracnose resistance locus Co-4 of common bean is located on chromosome 3 and contains putative disease resistance-related genes.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2004Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have constructed a common bean (Phaseolus vulgaris L.) bacterial artificial chromosome (BAC) library consisting of 33,792 clones and an estimated 3- to 5-fold coverage of the common bean genome. Leaf nuclei were used as the source for high-molecular-weight DNA, and an endonuclease/methylase competition assay was employed to partially cleave the DNA. The library was screened with a number of nuclear and mitochondrial probes. Each nuclear probe identified at least two BACs with an average insert size of ca. 100 kb. Only 26 clones were identified after hybridizing with mitochondrial probes, indicating contamination with organellar sequences is low. Numerous clones could be identified after screening the library with two repetitive probes flanking the nuclear fertility restorer Fr. Intriguingly, 12 clones appeared to hybridize to both markers, and restriction analysis of these clones revealed that they can be assembled into maximally four contigs, suggesting that these repetitive probes may be useful for the physical mapping of the Fr locus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.