ArticleMolecular and cellular biology1999
Structural and functional cross-talk between a distant enhancer and the epsilon-globin gene promoter shows interdependence of the two elements in chromatin.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements.Nature methods · 2015Article
- The role of transcriptional activator GATA-1 at human beta-globin HS2.Nucleic acids research · 2008Article
- Beta-globin intergenic transcription and histone acetylation dependent on an enhancer.Molecular and cellular biology · 2007Article
- Nucleosome and transcription activator antagonism at human beta-globin locus control region DNase I hypersensitive sites.Nucleic acids research · 2007Article
- Recruitment of transcription complexes to the beta-globin gene locus in vivo and in vitro.The Journal of biological chemistry · 2004Article
- Bystander gene activation by a locus control region.The EMBO journal · 2004Article
- An insulator blocks spreading of histone acetylation and interferes with RNA polymerase II transfer between an enhancer and gene.Nucleic acids research · 2004Article
- A major role for the TATA box in recruitment of chromatin modifying complexes to a globin gene promoter.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Beta-globin locus control region HS2 and HS3 interact structurally and functionally.Nucleic acids research · 2003Article
- Role of NF-Y in in vivo regulation of the gamma-globin gene.Molecular and cellular biology · 2001Article
- Acetylation of a specific promoter nucleosome accompanies activation of the epsilon-globin gene by beta-globin locus control region HS2.Molecular and cellular biology · 2001Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the requirements for enhancer-promoter communication by using the human beta-globin locus control region (LCR) DNase I-hypersensitive site 2 (HS2) enhancer and the epsilon-globin gene in chromatinized minichromosomes in erythroid cells. Activation of globin genes during development is accompanied by localized alterations of chromatin structure, and CACCC binding factors and GATA-1, which interact with both globin promoters and the LCR, are believed to be critical for globin gene transcription activation. We found that an HS2 element mutated in its GATA motif failed to remodel the epsilon-globin promoter or activate transcription yet HS2 nuclease accessibility did not change. Accessibility and transcription were reduced at promoters with mutated GATA-1 or CACCC sites. Strikingly, these mutations also resulted in reduced accessibility at HS2. In the absence of a globin gene, HS2 is similarly resistant to nuclease digestion. In contrast to observations in Saccharomyces cerevisiae, HS2-dependent promoter remodeling was diminished when we mutated the TATA box, crippling transcription. This mutation also reduced HS2 accessibility. The results indicate that the epsilon-globin promoter and HS2 interact both structurally and functionally and that both upstream activators and the basal transcription apparatus contribute to the interaction. Further, at least in this instance, transcription activation and promoter remodeling by a distant enhancer are not separable.
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