ArticleMolecular and cellular biology1999
The Rpb4 subunit of fission yeast Schizosaccharomyces pombe RNA polymerase II is essential for cell viability and similar in structure to the corresponding subunits of higher eukaryotes.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 58 citations in OpenAlex.
- Structural Characterization of Native RNA Polymerase II Transcription Complexes and Nucleosomes in Drosophila melanogaster.Nature communications · 2026Article
- Gold nanoparticles and tilt pairs to assess protein flexibility by cryo-electron microscopy.Ultramicroscopy · 2021Article
- RNA polymerase II subunit D is essential for zebrafish development.Scientific reports · 2020Article
- Sub1 contacts the RNA polymerase II stalk to modulate mRNA synthesis.Nucleic acids research · 2017Article
- The dissociable RPB4 subunit of RNA Pol II has vital functions in Drosophila.Molecular genetics and genomics : MGG · 2010Article
- In vitro interaction between the N-terminus of the Ewing's sarcoma protein and the subunit of RNA polymerase II hsRPB7.Molecular biology reports · 2009Article
- Genome-associated RNA polymerase II includes the dissociable Rpb4/7 subcomplex.The Journal of biological chemistry · 2008Article
- Basal transcription machinery: role in regulation of stress response in eukaryotes.Journal of biosciences · 2007Review
- The fission yeast Rpb4 subunit of RNA polymerase II plays a specialized role in cell separation.Molecular genetics and genomics : MGG · 2006Article
- Fcp1 directly recognizes the C-terminal domain (CTD) and interacts with a site on RNA polymerase II distinct from the CTD.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The homologous Drosophila transcriptional adaptors ADA2a and ADA2b are both required for normal development but have different functions.Molecular and cellular biology · 2005Article
- RNA polymerase II transcription apparatus in Schizosaccharomyces pombe.Current genetics · 2004Review
- Domainal organization of the lower eukaryotic homologs of the yeast RNA polymerase II core subunit Rpb7 reflects functional conservation.Nucleic acids research · 2004Article
- Rpb7 subunit of RNA polymerase II interacts with an RNA-binding protein involved in processing of transcripts.Nucleic acids research · 2003Article
- Architecture of initiation-competent 12-subunit RNA polymerase II.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- An Rpb4/Rpb7-like complex in yeast RNA polymerase III contains the orthologue of mammalian CGRP-RCP.Molecular and cellular biology · 2003Article
- Identification of histone H4-like TAF in Schizosaccharomyces pombe as a protein that interacts with WD repeat-containing TAF.Nucleic acids research · 2002Article
- Formation of a carboxy-terminal domain phosphatase (Fcp1)/TFIIF/RNA polymerase II (pol II) complex in Schizosaccharomyces pombe involves direct interaction between Fcp1 and the Rpb4 subunit of pol II.Molecular and cellular biology · 2002Article
- Article
- Multiple mechanisms of suppression circumvent transcription defects in an RNA polymerase mutant.Molecular and cellular biology · 2000Article
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4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Both the gene and the cDNA encoding the Rpb4 subunit of RNA polymerase II were cloned from the fission yeast Schizosaccharomyces pombe. The cDNA sequence indicates that Rpb4 consists of 135 amino acid residues with a molecular weight of 15,362. As in the case of the corresponding subunits from higher eukaryotes such as humans and the plant Arabidopsis thaliana, Rpb4 is smaller than RPB4 from the budding yeast Saccharomyces cerevisiae and lacks several segments, which are present in the S. cerevisiae RPB4 subunit, including the highly charged sequence in the central portion. The RPB4 subunit of S. cerevisiae is not essential for normal cell growth but is required for cell viability under stress conditions. In contrast, S. pombe Rpb4 was found to be essential even under normal growth conditions. The fraction of RNA polymerase II containing RPB4 in exponentially growing cells of S. cerevisiae is about 20%, but S. pombe RNA polymerase II contains the stoichiometric amount of Rpb4 even at the exponential growth phase. In contrast to the RPB4 homologues from higher eukaryotes, however, S. pombe Rpb4 formed stable hybrid heterodimers with S. cerevisiae RPB7, suggesting that S. pombe Rpb4 is similar, in its structure and essential role in cell viability, to the corresponding subunits from higher eukaryotes. However, S. pombe Rpb4 is closer in certain molecular functions to S. cerevisiae RPB4 than the eukaryotic RPB4 homologues.
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