ArticleMolecular and cellular biology1999
In vivo analysis of functional regions within yeast Rap1p.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- BRCT Domains: Structure, Functions, and Implications in Disease-New Therapeutic Targets for Innovative Drug Discovery against Infections.Pharmaceutics · 2023Review
- Article
- Structural basis of G-quadruplex DNA recognition by the yeast telomeric protein Rap1.Nucleic acids research · 2020Article
- Perturbing proteomes at single residue resolution using base editing.Nature communications · 2020Article
- Trypanosoma brucei RAP1 Has Essential Functional Domains That Are Required for Different Protein Interactions.mSphere · 2020Article
- Identification of a transcriptional activation domain in yeast repressor activator protein 1 (Rap1) using an altered DNA-binding specificity variant.The Journal of biological chemistry · 2017Article
- Alternative arrangements of telomeric recognition sites regulate the binding mode of the DNA-binding domain of yeast Rap1.Biophysical chemistry · 2015Article
- TFIIS is required for the balanced expression of the genes encoding ribosomal components under transcriptional stress.Nucleic acids research · 2012Article
- Everything you ever wanted to know about Saccharomyces cerevisiae telomeres: beginning to end.Genetics · 2012Review
- Genomic insights into the different layers of gene regulation in yeast.Genetics research international · 2011Article
- Regulon-specific control of transcription elongation across the yeast genome.PLoS genetics · 2009Article
- Microarray profiling of phage-display selections for rapid mapping of transcription factor-DNA interactions.PLoS genetics · 2009Article
- A conserved acidic patch in the Myb domain is required for activation of an endogenous target gene and for chromatin binding.Molecular cancer · 2008Article
- The transcriptional repressor activator protein Rap1p is a direct regulator of TATA-binding protein.The Journal of biological chemistry · 2008Article
- Yeast TFIID serves as a coactivator for Rap1p by direct protein-protein interaction.Molecular and cellular biology · 2007Article
- Conservation and evolvability in regulatory networks: the evolution of ribosomal regulation in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Counting of Rif1p and Rif2p on Saccharomyces cerevisiae telomeres regulates telomere length.Molecular and cellular biology · 2004Article
- Comparison of ABF1 and RAP1 in chromatin opening and transactivator potentiation in the budding yeast Saccharomyces cerevisiae.Molecular and cellular biology · 2004Article
- The different (sur)faces of Rap1p.Molecular genetics and genomics : MGG · 2003Review
- Rrb1p, a yeast nuclear WD-repeat protein involved in the regulation of ribosome biosynthesis.Molecular and cellular biology · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have analyzed the in vivo importance of different regions of Rap1p, a yeast transcriptional regulator and telomere binding protein. A yeast strain (SCR101) containing a regulatable RAP1 gene was used to test functional complementation by a range of Rap1p derivatives. These experiments demonstrated that the C terminus of the protein, containing the putative transcriptional activation domain and the regions involved in silencing and telomere function, is not absolutely essential for cell growth, a result confirmed by sporulation of a diploid strain containing a C terminal deletion derivative of RAP1. Northern analysis with cells that expressed Rap1p lacking the transcriptional activation domain revealed that this region is important for the expression of only a subset of Rap1p-activated genes. The one essential region within Rap1p is the DNA binding domain. We have investigated the possibility that this region has additional functions. It contains two Myb-like subdomains separated by a linker region. Individual point mutations in the linker region had no effect on Rap1p function, although deletion of the region abolished cell growth. The second Myb-like subdomain contains a large unstructured loop of unknown function. Domain swap experiments with combinations of elements from DNA binding domains of Rap1p homologues from different yeasts revealed that major changes can be made to the amino acid composition of this region without affecting Rap1p function.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.