Evidence map›Paper›PMID 10523624›Full record

ArticleMolecular and cellular biology1999

A sampling of the yeast proteome.

B Futcher, G I Latter, P Monardo, C S McLaughlin, J I Garrels

Open access · bronzeAbstract read
In one paragraph

Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 201 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
201citing papers in PubMed, 1 pooled it
11.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

201 citing papers in PubMed, 1 synthesis or guideline pooled it, 616 citations in OpenAlex.

  1. Pooled it
  2. Trial
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Estimating Total Quantitative Protein Content inInternational journal of molecular sciences · 2023
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Review
  19. Application of Solid-State Nanopore in Protein Detection.International journal of molecular sciences · 2020
    Review
  20. Expanding Role of Ubiquitin in Translational Control.International journal of molecular sciences · 2020
    Review

141 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

B FutcherCold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA. futcher@cshl.org
G I Latter
P Monardo
C S McLaughlin
J I Garrels
Cold Spring Harbor Laboratory · USProteome Sciences (United Kingdom) · GBUniversity of California, Irvine · US

Funding

RESOURCE FOR CONSTRUCTION OF 2D GEL PROTEIN DATABASESP41RR002188 · NCRR · COLD SPRING HARBOR LABORATORY · PI LATTER, GERALD · 1985 to 1994
–
WIN CELL CYCLE CLOCKS IN S CEREVISIAER01GM045410 · NIGMS · COLD SPRING HARBOR LABORATORY · PI FUTCHER, ALLEN BRUCE · 1991 to 1999
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COMPREHENSIVE PROTEIN DATABASE FOR YEAST S CEREVISIAER44GM054110 · NIGMS · PROTEOME, INC. · PI GARRELS, JAMES I · 1997 to 1998
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NCRR NIH HHS P41-RR02188NIGMS NIH HHS R01 GM45410NIGMS NIH HHS R44 GM54110
6 · The paper itself

Abstract

In this study, we examined yeast proteins by two-dimensional (2D) gel electrophoresis and gathered quantitative information from about 1,400 spots. We found that there is an enormous range of protein abundance and, for identified spots, a good correlation between protein abundance, mRNA abundance, and codon bias. For each molecule of well-translated mRNA, there were about 4,000 molecules of protein. The relative abundance of proteins was measured in glucose and ethanol media. Protein turnover was examined and found to be insignificant for abundant proteins. Some phosphoproteins were identified. The behavior of proteins in differential centrifugation experiments was examined. Such experiments with 2D gels can give a global view of the yeast proteome.

Indexed as

Gene Expression ProfilingCodonElectrophoresis, Gel, Two-DimensionalFungal ProteinsGenes, FungalGenetic CodeImage Processing, Computer-AssistedProtein BiosynthesisRNA, FungalRNA, MessengerSaccharomyces cerevisiaeCodonFungal ProteinsRNA, FungalRNA, Messenger

Identifiers

PMID10523624
PMCPMC84729
OpenAlexW2150566147

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.