ArticleMolecular and cellular biology1999
An open reading frame element mediates posttranscriptional regulation of tropoelastin and responsiveness to transforming growth factor beta1.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 63 citations in OpenAlex.
- RiboScreenInternational journal of molecular sciences · 2024Article
- Emerging mechanisms of elastin transcriptional regulation.American journal of physiology. Cell physiology · 2022Review
- Identification and characterization of novel elastin gene mutations in eleven families with supravalvular aortic stenosis.Frontiers in genetics · 2022Article
- Functional Vascular Tissue Engineering Inspired by Matricellular Proteins.Frontiers in cardiovascular medicine · 2019Review
- Matrix metalloproteinases in emphysema.Matrix biology : journal of the International Society for Matrix Biology · 2018Review
- Elastin-driven genetic diseases.Matrix biology : journal of the International Society for Matrix Biology · 2018Review
- Elastin, arterial mechanics, and cardiovascular disease.American journal of physiology. Heart and circulatory physiology · 2018Review
- Codependence of Bone Morphogenetic Protein Receptor 2 and Transforming Growth Factor-β in Elastic Fiber Assembly and Its Perturbation in Pulmonary Arterial Hypertension.Arteriosclerosis, thrombosis, and vascular biology · 2017Article
- Design of an elastin-layered dermal regeneration template.Acta biomaterialia · 2017Article
- Expression of versican V3 by arterial smooth muscle cells alters tumor growth factor β (TGFβ)-, epidermal growth factor (EGF)-, and nuclear factor κB (NFκB)-dependent signaling pathways, creating a microenvironment that resists monocyte adhesion.The Journal of biological chemistry · 2014Article
- A cytokine axis regulates elastin formation and degradation.Matrix biology : journal of the International Society for Matrix Biology · 2013Review
- Aspartic acid racemisation in purified elastin from arteries as basis for age estimation.International journal of legal medicine · 2010Article
- Tissue elasticity and the ageing elastic fibre.Age (Dordrecht, Netherlands) · 2009Review
- The role of caveolin-1 in pulmonary matrix remodeling and mechanical properties.American journal of physiology. Lung cellular and molecular physiology · 2008Article
- EGF antagonizes TGF-beta-induced tropoelastin expression in lung fibroblasts via stabilization of Smad corepressor TGIF.American journal of physiology. Lung cellular and molecular physiology · 2008Article
- Competency for nonsense-mediated reduction in collagen X mRNA is specified by the 3' UTR and corresponds to the position of mutations in Schmid metaphyseal chondrodysplasia.American journal of human genetics · 2008Article
- Regulation of luteinizing hormone receptor mRNA expression by a specific RNA binding protein in the ovary.Molecular and cellular endocrinology · 2007Article
- Identification, structural characterization, and tissue distribution of Tsg-5: a new TNF-stimulated gene.Genes and immunity · 2003Article
- Age estimation based on aspartic acid racemization in elastin from the yellow ligaments.International journal of legal medicine · 2003Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Elastin, an extracellular component of arteries, lung, and skin, is produced during fetal and neonatal growth. We reported previously that the cessation of elastin production is controlled by a posttranscriptional mechanism. Although tropoelastin pre-mRNA is transcribed at the same rate in neonates and adults, marked instability of the fully processed transcript bars protein production in mature tissue. Using RNase protection, we identified a 10-nucleotide sequence in tropoelastin mRNA near the 5' end of the sequences coded by exon 30 that interacts specifically with a developmentally regulated cytosolic 50-kDa protein. Binding activity increased as tropoelastin expression dropped, being low in neonatal fibroblasts and high in adult cells, and treatment with transforming growth factor beta1 (TGF-beta1), which stimulates tropoelastin expression by stabilizing its mRNA, reduced mRNA-binding activity. No other region of tropoelastin mRNA interacted with cellular proteins, and no binding activity was detected in nuclear extracts. The ability of the exon-30 element to control mRNA decay and responsiveness to TGF-beta1 was assessed by three distinct functional assays: (i) insertion of exon 30 into a heterologous gene conferred increased reporter activity after exposure to TGF-beta1; (ii) addition of excess exon 30 RNA slowed tropoelastin mRNA decay in an in vitro polysome degradation assay; and (iii) a mutant tropoelastin cDNA lacking exon 30, compared to wild-type cDNA, produced a stable transcript whose levels were not affected by TGF-beta1. These findings demonstrate that posttranscriptional regulation of elastin production in mature tissue is conferred by a specific element within the open reading frame of tropoelastin mRNA.
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