ArticleJournal of clinical microbiology1999
Detection of human herpesvirus 6 by reverse transcription-PCR.
Article in Journal of clinical microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 87 citations in OpenAlex.
- A customizable multiplex protein microarray for antibody testing and its application for tick-borne and other infectious diseases.Scientific reports · 2025Article
- Fetal cord plasma herpesviruses and preeclampsia: an observational cohort study.Scientific reports · 2024Observational
- Effects of Psychological and Physical Stress on Oxidative Stress, Serotonin, and Fatigue in Young Females Induced by Objective Structured Clinical Examination: Pilot Study of u-8-OHdG, u-5HT, and s-HHV-6.International journal of tryptophan research : IJTR · 2021Article
- RNA Sequencing of theJournal of virology · 2019Article
- Comparative genomic, transcriptomic, and proteomic reannotation of human herpesvirus 6.BMC genomics · 2018Article
- A Celebration of the Life and Work of Caroline Breese Hall, MD.Journal of the Pediatric Infectious Diseases Society · 2016Article
- Past, present, and future perspectives on the diagnosis of Roseolovirus infections.Current opinion in virology · 2014Review
- Article
- Prevalence of human Herpesviridae in cerebrospinal fluid of patients with multiple sclerosis and noninfectious neurological disease in the Netherlands.Journal of neurovirology · 2014Article
- Persistent human herpesvirus-6 infection in patients with an inherited form of the virus.Journal of medical virology · 2013Article
- Article
- Diagnostic assays for active infection with human herpesvirus 6 (HHV-6).Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2010Article
- The associations between psychosocial stress and the frequency of illness, and innate and adaptive immune function in children.Brain, behavior, and immunity · 2008Article
- Beta-herpesviruses in febrile children with cancer.Emerging infectious diseases · 2008Article
- Update on human herpesvirus 6 biology, clinical features, and therapy.Clinical microbiology reviews · 2005Review
- Human Herpesvirus 6 Infection of the Central Nervous System.Current infectious disease reports · 2004Article
- Real-time quantitative PCR for human herpesvirus 6 DNA.Journal of clinical microbiology · 2000Article
- An immunoblot assay for detection of immunoglobulin M antibody to human herpesvirus 6.Clinical and diagnostic laboratory immunology · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The role of human herpesvirus 6 (HHV-6) in disease beyond primary infection remains unclear. We have developed and validated a new reverse transcription-PCR (RT-PCR) assay for HHV-6 that can determine the presence of HHV-6 in clinical specimens and differentiate between latent and replicating virus. Peripheral blood mononuclear cells from 109 children were evaluated for HHV-6 by RT-PCR, DNA PCR, and viral culture. Of these samples, 106 were suitable for analysis. A total of 20 samples were positive for HHV-6 by culture and DNA PCR, of which 19 were positive by RT-PCR (sensitivity, 95%). All 28 samples from children that were negative by viral culture, but positive by DNA PCR, were negative for viral transcripts by our RT-PCR assay. One positive RT-PCR result was observed in 56 samples that were negative by tissue culture and DNA PCR. This indicates a low rate of false-positive results (1.2%) and a specificity of 98.8%. This RT-PCR assay can reliably differentiate between latent and actively replicating HHV-6 and should allow insight into the pathogenesis of this ubiquitous virus.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.