Evidence map›Paper›PMID 10523524›Full record

ArticleGenome research1999

Generation and analysis of 25 Mb of genomic DNA from the pufferfish Fugu rubripes by sequence scanning.

G Elgar, M S Clark, S Meek, S Smith, S Warner, Y J Edwards, N Bouchireb, A Cottage, G S Yeo, Y Umrania and 2 more

Abstract read
In one paragraph

Article in Genome research, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed.

  1. Insertion Hot Spots ofInternational journal of molecular sciences · 2019
    Article
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  5. Analysis of transposons and repeat composition of the sunflower (Helianthus annuus L.) genome.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010
    Article
  6. Article
  7. A genome-wide microsatellite polymorphism database for the indica and japonica rice.DNA research : an international journal for rapid publication of reports on genes and genomes · 2007
    Article
  8. Article
  9. Article
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  16. Evolution of signal transduction by gene and genome duplication in fish.Journal of structural and functional genomics · 2003
    Review
  17. Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

G ElgarUK Human Genome Mapping Project (HGMP) Resource Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SB, UK. gelgar@hgmp.mrc.ac.uk
M S Clark
S Meek
S Smith
S Warner
Y J Edwards
N Bouchireb
A Cottage
G S Yeo
Y Umrania
G Williams
S Brenner

Funding

Wellcome Trust
6 · The paper itself

Abstract

We have generated and analyzed >50,000 shotgun clones from 1059 Fugu cosmid clones. All sequences have been minimally edited and searched against protein and DNA databases. These data are all displayed on a searchable, publicly available web site at. With an average of 50 reads per cosmid, this is virtually nonredundant sequence skimming, covering 30%-50% of each clone. This essentially random data set covers nearly 25 Mb (>6%) of the Fugu genome and forms the basis of a series of whole genome analyses which address questions regarding gene density and distribution in the Fugu genome and the similarity between Fugu and mammalian genes. The Fugu genome, with eight times less DNA but a similar gene repertoire, is ideally suited to this type of study because most cosmids contain more than one identifiable gene. General features of the genome are also discussed. We have made some estimation of the syntenic relationship between mammals and Fugu and looked at the efficacy of ORF prediction from short, unedited Fugu genomic sequences. Comparative DNA sequence analyses are an essential tool in the functional interpretation of complex vertebrate genomes. This project highlights the utility of using the Fugu genome in this kind of study.

Indexed as

GenomeMicrosatellite RepeatsAnimalsBase SequenceCosmidsDatabases, FactualDNA, SatelliteExonsFishes, PoisonousIntronsModels, GeneticMolecular Sequence DataOpen Reading FramesSequence Analysis, DNADNA, Satellite

Identifiers

PMID10523524
PMCPMC310822

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.