ArticleProceedings of the National Academy of Sciences of the United States of America1999
Continuous detection of extracellular ATP on living cells by using atomic force microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 76 citations in OpenAlex.
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- A Rapid Unraveling of the Activity and Antibiotic Susceptibility of Mycobacteria.Antimicrobial agents and chemotherapy · 2019Article
- The endothelial glycocalyx anchors von Willebrand factor fibers to the vascular endothelium.Blood advances · 2018Article
- Use of luciferase probes to measure ATP in living cells and animals.Nature protocols · 2017Article
- Review
- Shear stress induces a longitudinal Ca(2+) wave via autocrine activation of P2Y1 purinergic signalling in rat atrial myocytes.The Journal of physiology · 2015Article
- Detecting nanoscale vibrations as signature of life.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Real-time monitoring of protein conformational changes using a nano-mechanical sensor.PloS one · 2014Article
- Recent advances in application of biosensors in tissue engineering.BioMed research international · 2014Review
- Detection of endogenous substances with enzymatic microelectrode biosensors in the kidney.American journal of physiology. Regulatory, integrative and comparative physiology · 2013Review
- Rapid detection of bacterial resistance to antibiotics using AFM cantilevers as nanomechanical sensors.Nature nanotechnology · 2013Article
- Detecting adenosine triphosphate in the pericellular space.Interface focus · 2013Article
- Optical ATP biosensor for extracellular ATP measurement.Biosensors & bioelectronics · 2013Article
- ATP release from non-excitable cells.Purinergic signalling · 2009Article
- Defective acid sphingomyelinase pathway with Pseudomonas aeruginosa infection in cystic fibrosis.American journal of respiratory cell and molecular biology · 2009Article
- Role of mechanical stress in regulating airway surface hydration and mucus clearance rates.Respiratory physiology & neurobiology · 2008Review
- Cyclic AMP regulates bicarbonate secretion in cholangiocytes through release of ATP into bile.Gastroenterology · 2007Article
- Physiological regulation of ATP release at the apical surface of human airway epithelia.The Journal of biological chemistry · 2006Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Atomic force microscopy is a powerful technique used to investigate the surface of living cells under physiological conditions. The resolution of the instrument is mainly limited by the softness of living cells and the interactions with the scanning tip (cantilever). Atomic force microscopy, in combination with myosin-functionalized cantilevers, was used in the detection of ATP concentrations in solution and on living cells. Functionally active tips were used to scan the surface of cells in culture and to show that the CFTR+ cell line (S9) had a basal surface ATP concentration that could be detected with atomic force microscopy (n = 10). ATP-dependent signals were not detectable in cells scanned with noncoated or heat-inactivated enzyme-coated tips (n = 9). Enzymatically active tips may serve as a model for future development of atomic force microscopy biosensors that can simultaneously detect topographical and biologically important compounds at the surface microenvironment of living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.