ArticleProceedings of the National Academy of Sciences of the United States of America1999
Productive infection of double-negative T cells with HIV in vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Productive human immunodeficiency virus type 1 infection in peripheral blood predominantly takes place in CD4/CD8 double-negative T lymphocytes.Journal of virology · 2007Trial
- Article
- Article
- Dysregulated neuroimmune interactions and sustained type I interferon signaling after human immunodeficiency virus type 1 infection of human iPSC derived microglia and cerebral organoids.bioRxiv : the preprint server for biology · 2023Article
- Elevated Foxp3Frontiers in immunology · 2022Article
- Actin Polymerization Is Required for Filopodia Formation Supporting HSV-1 Entry into Activated T Cells.Current microbiology · 2021Article
- Conversion of effector CD4Cellular & molecular immunology · 2021Article
- HIV Infection and Persistence in Pulmonary Mucosal Double Negative T CellsJournal of virology · 2020Article
- Renal double negative T cells: unconventional cells in search of a function.Annals of translational medicine · 2019Article
- Article
- Concomitant Disruption ofJournal of immunology (Baltimore, Md. : 1950) · 2017Article
- Impact of the SCF signaling pathway on leukemia stem cell-mediated ATL initiation and progression in an HBZ transgenic mouse model.Oncotarget · 2016Article
- Double negative (DN) αβ T cells: misperception and overdue recognition.Immunology and cell biology · 2015Review
- Double-negative T cells during HIV/SIV infections: potential pinch hitters in the T-cell lineup.Current opinion in HIV and AIDS · 2012Review
- Multi-scale modeling of HIV infection in vitro and APOBEC3G-based anti-retroviral therapy.PLoS computational biology · 2012Article
- Host factors influencing susceptibility to HIV infection and AIDS progression.Retrovirology · 2007Review
- Human immunodeficiency virus type 1 Vpr induces DNA replication stress in vitro and in vivo.Journal of virology · 2006Article
- CD4-CD8- T cells control intracellular bacterial infections both in vitro and in vivo.The Journal of experimental medicine · 2005Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
HIV induces CD4 down-regulation from the surface of infected cells by several independent mechanisms, suggesting an important biological role for this phenomenon. In vitro CD4 down-regulation generates T cells with a double-negative (DN) CD4(-)CD8(-) T cell receptor-alphabeta(+) phenotype. However, evidence that this down-regulation occurs in vivo in HIV-infected subjects is lacking, and viral load or viral production assays invariably focus on CD4(+) T cells. We show here that HIV infection can often be detected in sorted DN cells from peripheral blood and lymph nodes, even when plasma viral load is undetectable. DN T cells infected with HIV represented up to 20% of the cellular viral load in T cells, as determined by DNA PCR. In patients on successful highly active antiretroviral therapy, the viral load decreased in the plasma in CD4(+) and in DN T cells, suggesting that infected DN cells, like CD4(+) cells, contribute to viral production and are sensitive to highly active antiretroviral therapy. Indeed, HIV unspliced and multispliced RNAs were often detectable in DN T cells in spite of the small size of this subset. Infectious virus from DN T cells was transmitted efficiently in coculture experiments with uninfected T cell lymphoblasts, even when viral DNA in the DN cells was barely detectable. We conclude that a discrete population of infected DN T cells exists in HIV-positive subjects, even when the plasma viral load is undetectable. These cells may represent an important source of infectious virus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.