ArticlePlant physiology1999
Biochemical and immunocytochemical characterization of two types of myosins in cultured tobacco bright yellow-2 cells
Article in Plant physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 52 citations in OpenAlex.
- Update on Myosin Motors: Molecular Mechanisms and Physiological Functions.Plant physiology · 2018Review
- Article
- Cytokinesis defect in BY-2 cells caused by ATP-competitive kinase inhibitors.Plant signaling & behavior · 2016Article
- The circular F-actin bundles provide a track for turnaround and bidirectional movement of mitochondria in Arabidopsis root hair.PloS one · 2014Article
- Calcium-induced mechanical change in the neck domain alters the activity of plant myosin XI.The Journal of biological chemistry · 2012Article
- Quantitative analysis of organelle distribution and dynamics in Physcomitrella patens protonemal cells.BMC plant biology · 2012Article
- Plant-Specific Myosin XI, a Molecular Perspective.Frontiers in plant science · 2012Article
- Characteristics of light chains of Chara myosin revealed by immunological investigation.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2012Article
- Myosin XI-dependent formation of tubular structures from endoplasmic reticulum isolated from tobacco cultured BY-2 cells.Plant physiology · 2011Article
- Myosin-dependent endoplasmic reticulum motility and F-actin organization in plant cells.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- An isoform of myosin XI is responsible for the translocation of endoplasmic reticulum in tobacco cultured BY-2 cells.Journal of experimental botany · 2009Article
- Truncated myosin XI tail fusions inhibit peroxisome, Golgi, and mitochondrial movement in tobacco leaf epidermal cells: a genetic tool for the next generation.Journal of experimental botany · 2008Article
- The sliding theory of cytoplasmic streaming: fifty years of progress.Journal of plant research · 2007Review
- Isolation of myosin XI genes from the Closterium peracerosum-strigosum-littorale complex and analysis of their expression during sexual reproduction.Journal of plant research · 2006Article
- Arabidopsis myosin XI mutant is defective in organelle movement and polar auxin transport.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Higher plant myosin XI moves processively on actin with 35 nm steps at high velocity.The EMBO journal · 2003Article
- Analysis of the myosins encoded in the recently completed Arabidopsis thaliana genome sequence.Genome biology · 2001Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have isolated a myosin (referred to as 170-kD myosin) from lily pollen tubes, which consists of 170-kD heavy chain and calmodulin (CaM) light chain and is responsible for cytoplasmic streaming. A 170-kD polypeptide that has similar antigenicity to the 170-kD myosin heavy chain of lily pollen tubes was also present in cultured tobacco (Nicotiana tabacum) Bright Yellow-2 (BY-2) cells, and possessed the ability to interact with F-actin in an ATP-dependent manner. In addition to this myosin, we identified biochemically another kind of myosin in BY-2 cells. This myosin consisted of a CaM light chain and a 175-kD heavy chain with antigenicity different from the 170-kD myosin heavy chain. In the present study, we referred to this myosin as 175-kD myosin. This myosin was able to translocate rhodamine-phalloidin (RP)-labeled F-actin at an average velocity of about 9 &mgr;m/s in the motility assay in vitro. In contrast, the sliding velocity of RP-labeled F-actin translocated by fractions containing the 170-kD myosin was 3 to 4 &mgr;m/s. The velocity of cytoplasmic streaming in living BY-2 cells ranged from 2 to 9 &mgr;m/s. The motile activity of 175-kD myosin in vitro was inhibited by Ca(2+) at concentrations higher than 10(-6) M. Immunoblot analyses using an antiserum against the heavy chain of 170- or 175-kD myosin revealed that in tobacco plants, the 175-kD myosin was expressed in leaf, stem, and root, but not in germinating pollen, while 170-kD myosin was present in all of these plant parts and in germinating pollen. These results suggest that the two types of myosins, 170 and 175 kD, presumably participate in cytoplasmic streaming in BY-2 cells and other somatic cells of tobacco plants.
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