ArticlePlant physiology1999
The enzymatic activity of fungal xylanase is not necessary for its elicitor activity.
Article in Plant physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 144 citations in OpenAlex.
- Glucuronoyl Esterase of Pathogenic Phanerochaete carnosa Induces Immune Responses in Aspen Independently of Its Enzymatic Activity.Plant biotechnology journal · 2026Article
- Plant cell walls as a key driver of plant-pathogen coevolution.FEMS microbiology reviews · 2026Review
- Genome-Wide Identification and Analysis of Carbohydrate-Binding Modules inInternational journal of molecular sciences · 2025Article
- A novel xylanase from a myxobacterium triggers a plant immune response in Nicotiana benthamiana.Molecular plant pathology · 2024Article
- Global characterization of GH11 family xylanases genes inFrontiers in microbiology · 2024Article
- Integrity of xylan backbone affects plant responses to drought.Frontiers in plant science · 2024Article
- Advances in Fungal Elicitor-Triggered Plant Immunity.International journal of molecular sciences · 2022Review
- TheInternational journal of molecular sciences · 2021Article
- Cytotoxic function of xylanase VdXyn4 in the plant vascular wilt pathogen Verticillium dahliae.Plant physiology · 2021Article
- The Role of Glycoside Hydrolases in Phytopathogenic Fungi and Oomycetes Virulence.International journal of molecular sciences · 2021Review
- FoEG1, a secreted glycoside hydrolase family 12 protein from Fusarium oxysporum, triggers cell death and modulates plant immunity.Molecular plant pathology · 2021Article
- Genome-Wide Identification ofFrontiers in plant science · 2021Article
- The Apoplast: A Key Player in Plant Survival.Antioxidants (Basel, Switzerland) · 2020Review
- The fungal subtilase AsES elicits a PTI-like defence response in Arabidopsis thaliana plants independently of its enzymatic activity.Molecular plant pathology · 2020Article
- Elicitor and Receptor Molecules: Orchestrators of Plant Defense and Immunity.International journal of molecular sciences · 2020Review
- Functional and chemical characterization of XAF: a heat-stable plant polymer that activates xyloglucan endotransglucosylase/hydrolase (XTH).Annals of botany · 2019Article
- Verticillium dahliae PevD1, an Alt a 1-like protein, targets cotton PR5-like protein and promotes fungal infection.Journal of experimental botany · 2019Article
- Recognition of Elicitors in Grapevine: From MAMP and DAMP Perception to Induced Resistance.Frontiers in plant science · 2019Review
- A 25-Residue Peptide FromFrontiers in plant science · 2019Article
- A mutation in an exoglucanase of Xanthomonas oryzae pv. oryzae, which confers an endo mode of activity, affects bacterial virulence, but not the induction of immune responses, in rice.Molecular plant pathology · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fungal xylanases from Trichoderma spp. are potent elicitors of defense responses in various plants. To determine whether enzymatic activity is necessary for elicitor activity, we used site-directed mutagenesis to reduce the catalytic activity of xylanase II from Trichoderma reesei. For this, the glutamic acid residue at position 210, which is part of the active center in this family of enzymes, was changed to either aspartic acid (E210D) or serine (E210S). Wild-type and mutated forms of xylanase II were expressed in yeast cells and purified to homogeneity. Compared with the wild-type form of xylanase II, E210D had >100-fold and E210S 1,000-fold lower enzymatic activity. In contrast, these mutated forms showed no comparable drop in elicitor activity. They fully stimulated medium alkalinization and ethylene biosynthesis in suspension-cultured tomato (Lycopersicon esculentum) cells, as well as hypersensitive necrosis in leaves of tomato and tobacco (Nicotiana tabacum) plants. These results provide direct evidence that enzyme activity is not necessary for elicitor activity of fungal xylanase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.