ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1999
Astroglial differentiation of cortical precursor cells triggered by activation of the cAMP-dependent signaling pathway.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 63 citations in OpenAlex.
- Article
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- Stem cell-derived brainstem mouse astrocytes obtain a neurotoxic phenotype in vitro upon neuroinflammation.Journal of inflammation (London, England) · 2023Article
- Calcium and activity-dependent signaling in the developing cerebral cortex.Development (Cambridge, England) · 2022Review
- The potential of mechanistic information organised within the AOP framework to increase regulatory uptake of the developmental neurotoxicity (DNT) in vitro battery of assays.Reproductive toxicology (Elmsford, N.Y.) · 2021Article
- Astroglial Mechanisms of Ketamine Action Include Reduced Mobility of Kir4.1-Carrying Vesicles.Neurochemical research · 2020Review
- Differentiation of Mesenchymal Stem Cells to Neuroglia: in the Context of Cell Signalling.Stem cell reviews and reports · 2019Review
- Pituitary Adenylate Cyclase-Activating Polypeptide: 30 Years in Research Spotlight and 600 Million Years in Service.Journal of clinical medicine · 2019Review
- Cyclic AMP signaling restricts activation and promotes maturation and antioxidant defenses in astrocytes.BMC genomics · 2016Article
- Foxg1 has an essential role in postnatal development of the dentate gyrus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- CXCL12 alone is insufficient for gliomagenesis in Nf1 mutant mice.Journal of neuroimmunology · 2010Article
- Cyclic AMP suppression is sufficient to induce gliomagenesis in a mouse model of neurofibromatosis-1.Cancer research · 2010Article
- Alexander disease mutant glial fibrillary acidic protein compromises glutamate transport in astrocytes.Journal of neuropathology and experimental neurology · 2010Article
- Disparate developmental neurotoxicants converge on the cyclic AMP signaling cascade, revealed by transcriptional profiles in vitro and in vivo.Brain research · 2010Article
- PACAP signaling to DREAM: a cAMP-dependent pathway that regulates cortical astrogliogenesis.Molecular neurobiology · 2009Review
- DREAM mediates cAMP-dependent, Ca2+-induced stimulation of GFAP gene expression and regulates cortical astrogliogenesis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Ameliorating the developmental neurotoxicity of chlorpyrifos: a mechanisms-based approach in PC12 cells.Environmental health perspectives · 2007Article
- Rapid effects of retinoic acid on CREB and ERK phosphorylation in neuronal cells.Molecular biology of the cell · 2004Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
In the developing brain, differentiation of neural precursors into neurons or glial cells occurs in response to neurotrophic factors acting on the cell surface. Intracellular signaling mechanisms that relay information to initiate differentiative responses of neural precursor cells are poorly understood. To investigate whether stimulation of the cAMP-dependent signaling pathway participates in differentiative responses of cells in the developing CNS, we performed experiments using both conditionally immortalized neural precursor cells (RC2.E10 cells) and primary cultures of cells from developing rat cortex. Initially, we determined that RC2.E10 cells retain phenotypic features of neural precursors after inactivation of the immortalizing oncogene, a temperature-sensitive mutant of the simian virus 40 large-T antigen (SV40T). We found that, once SV40T is inactivated, RC2.E10 cells cease to divide and die. However, RC2. E10 cells can proliferate in the presence of basic fibroblast growth factor. In addition, they express nestin, a marker of neural precursor cells. Both RC2.E10 cells and primary cortical precursor cells undergo astroglial differentiation in response to cAMP stimulation by treatment with 8-bromo-cAMP. In both cases, cAMP-induced astrocyte differentiation is characterized by morphological changes, stimulation of glial fibrillary acidic protein expression, downregulation of nestin expression, and decreased proliferation. No increases in the expression of neuronal or oligodendrocytic markers were observed. Our results support the notion that the developing CNS contains neural precursor cells with the capacity of undergoing astrocyte differentiation in response to increased intracellular cAMP concentrations.
Indexed as
Identifiers
10516318PMC6782746W1584818544What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.