ArticleJournal of bacteriology1999
Functional analysis of glycosyltransferase genes from Lactococcus lactis and other gram-positive cocci: complementation, expression, and diversity.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 111 citations in OpenAlex.
- Linking exocellular polysaccharide structures and biosynthetic genes in lactic acid bacteria by combinatorial optimization.Applied and environmental microbiology · 2026Article
- Host-associatedCurrent research in food science · 2026Article
- Genomic and Transcriptomic Analyses Identify Two Key Glycosyltransferase GenesMicroorganisms · 2024Article
- New progress in the identifying regulatory factors of exopolysaccharide synthesis in lactic acid bacteria.World journal of microbiology & biotechnology · 2023Review
- Evolved distal tail protein of skunaviruses facilitates adsorption to exopolysaccharide-encoding lactococci.Microbiome research reports · 2023Article
- Effects of galactosyltransferase on EPS biosynthesis and freeze-drying resistance ofFood chemistry. Molecular sciences · 2022Article
- Agl24 is an ancient archaeal homolog of the eukaryotic N-glycan chitobiose synthesis enzymes.eLife · 2022Article
- Host-encoded, cell surface-associated exopolysaccharide required for adsorption and infection by lactococcal P335 phage subtypes.Frontiers in microbiology · 2022Article
- Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods.Foods (Basel, Switzerland) · 2021Review
- Article
- Genome editing using the endogenous type I CRISPR-Cas system inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Systems Biology - A Guide for Understanding and Developing Improved Strains of Lactic Acid Bacteria.Frontiers in microbiology · 2019Review
- Characterization ofFrontiers in microbiology · 2018Article
- Molecular Cloning, Expression and Characterization of Oenococcus oeni Priming Glycosyltransferases.Molecular biotechnology · 2017Article
- New Insights into Various Production Characteristics of Streptococcus thermophilus Strains.International journal of molecular sciences · 2016Review
- Biopolymers from lactic acid bacteria. Novel applications in foods and beverages.Frontiers in microbiology · 2015Review
- Bacterial exopolysaccharides: biosynthesis pathways and engineering strategies.Frontiers in microbiology · 2015Review
- Bifidobacterial surface-exopolysaccharide facilitates commensal-host interaction through immune modulation and pathogen protection.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Genome sequence of Lentisphaera araneosa HTCC2155T, the type species of the order Lentisphaerales in the phylum Lentisphaerae.Journal of bacteriology · 2010Article
- Cell surface of Lactococcus lactis is covered by a protective polysaccharide pellicle.The Journal of biological chemistry · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sixteen exopolysaccharide (EPS)-producing Lactococcus lactis strains were analyzed for the chemical compositions of their EPSs and the locations, sequences, and organization of the eps genes involved in EPS biosynthesis. This allowed the grouping of these strains into three major groups, representatives of which were studied in detail. Previously, we have characterized the eps gene cluster of strain NIZO B40 (group I) and determined the function of three of its glycosyltransferase (GTF) genes. Fragments of the eps gene clusters of strains NIZO B35 (group II) and NIZO B891 (group III) were cloned, and these encoded the NIZO B35 priming galactosyltransferase, the NIZO B891 priming glucosyltransferase, and the NIZO B891 galactosyltransferase involved in the second step of repeating-unit synthesis. The NIZO B40 priming glucosyltransferase gene epsD was replaced with an erythromycin resistance gene, and this resulted in loss of EPS production. This epsD deletion was complemented with priming GTF genes from gram-positive organisms with known function and substrate specificity. Although no EPS production was found with priming galactosyltransferase genes from L. lactis or Streptococcus thermophilus, complementation with priming glucosyltransferase genes involved in L. lactis EPS and Streptococcus pneumoniae capsule biosynthesis could completely restore or even increase EPS production in L. lactis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.