ArticleJournal of bacteriology1999
A plant-type (beta-class) carbonic anhydrase in the thermophilic methanoarchaeon Methanobacterium thermoautotrophicum.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 167 citations in OpenAlex.
- Introduction to Carbonic Anhydrase.Sub-cellular biochemistry · 2026Review
- Structural and functional characterization of a metagenomically derived γ-type carbonic anhydrase and its engineering into a hyperthermostable esterase.Protein science : a publication of the Protein Society · 2025Article
- Machine learning-guided discovery of thermophilic carbonic anhydrases from environmental metagenomes.Scientific reports · 2025Article
- Characterization of fungal carbonyl sulfide hydrolase belonging to clade D β-carbonic anhydrase.FEBS letters · 2025Article
- Highly Active Carbonic Anhydrase of the Thylakoid Lumen ofPlants (Basel, Switzerland) · 2024Article
- Biomimetic COEnvironmental science & technology · 2024Article
- Bio-integrated carbon capture and utilization: at the interface between capture chemistry and archaeal CONature communications · 2024Review
- Mineralogy of Bioprecipitate Evolution over Induction Times Mediated by Halophilic Bacteria under Various Mg/Ca Molar Ratios.ACS omega · 2022Article
- Comparison of Carbonic Anhydrases for COInternational journal of molecular sciences · 2022Article
- Carbon dioxide equivalent emissions from corn silage fermentation.Frontiers in microbiology · 2022Article
- Molecular Evidence for an Active Microbial Methane Cycle in Subsurface Serpentinite-Hosted Groundwaters in the Samail Ophiolite, Oman.Applied and environmental microbiology · 2021Article
- Selective Adsorption of Amino Acids in Crystals of Monohydrocalcite Induced by the Facultative AnaerobicFrontiers in microbiology · 2021Article
- High-level production in a plant system of a thermostable carbonic anhydrase and its immobilization on microcrystalline cellulose beads for COPlant cell reports · 2020Article
- Crystal Structure of β-Carbonic Anhydrase CafA from the Fungal PathogenMolecules and cells · 2020Article
- Carbonic anhydrase modification for carbon management.Environmental science and pollution research international · 2020Article
- The carbonic anhydrase of Clostridium autoethanogenum represents a new subclass of β-carbonic anhydrases.Applied microbiology and biotechnology · 2019Article
- Archaeal Communities in Deep Terrestrial Subsurface Underneath the Deccan Traps, India.Frontiers in microbiology · 2019Article
- Bacterial Community Dynamics and Biocement Formation during Stimulation and Augmentation: Implications for Soil Consolidation.Frontiers in microbiology · 2017Article
- Engineering de novo disulfide bond in bacterial α-type carbonic anhydrase for thermostable carbon sequestration.Scientific reports · 2016Article
- Thermostable Carbonic Anhydrases in Biotechnological Applications.International journal of molecular sciences · 2015Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Carbonic anhydrase, a zinc enzyme catalyzing the interconversion of carbon dioxide and bicarbonate, is nearly ubiquitous in the tissues of highly evolved eukaryotes. Here we report on the first known plant-type (beta-class) carbonic anhydrase in the archaea. The Methanobacterium thermoautotrophicum DeltaH cab gene was hyperexpressed in Escherichia coli, and the heterologously produced protein was purified 13-fold to apparent homogeneity. The enzyme, designated Cab, is thermostable at temperatures up to 75 degrees C. No esterase activity was detected with p-phenylacetate as the substrate. The enzyme is an apparent tetramer containing approximately one zinc per subunit, as determined by plasma emission spectroscopy. Cab has a CO(2) hydration activity with a k(cat) of 1.7 x 10(4) s(-1) and K(m) for CO(2) of 2.9 mM at pH 8.5 and 25 degrees C. Western blot analysis indicates that Cab (beta class) is expressed in M. thermoautotrophicum; moreover, a protein cross-reacting to antiserum raised against the gamma carbonic anhydrase from Methanosarcina thermophila was detected. These results show that beta-class carbonic anhydrases extend not only into the Archaea domain but also into the thermophilic prokaryotes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.