ArticleJournal of bacteriology1999
Genetic and biochemical characterization of a high-affinity betaine uptake system (BusA) in Lactococcus lactis reveals a new functional organization within bacterial ABC transporters.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 95 citations in OpenAlex.
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- Strategies to enhance stress tolerance in lactic acid bacteria across diverse stress conditions.World journal of microbiology & biotechnology · 2024Review
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- Health-Promoting Nature ofFrontiers in microbiology · 2022Article
- Evaluation and Proteomic Analysis of Lead Adsorption by Lactic Acid Bacteria.International journal of molecular sciences · 2019Article
- Identification of a GntR family regulator BusRExtremophiles : life under extreme conditions · 2019Article
- OpuF, a New Bacillus Compatible Solute ABC Transporter with a Substrate-Binding Protein Fused to the Transmembrane Domain.Applied and environmental microbiology · 2018Article
- Cyclic di-AMP regulation of osmotic homeostasis is essential in Group B Streptococcus.PLoS genetics · 2018Article
- The GbsR Family of Transcriptional Regulators: Functional Characterization of the OpuAR Repressor.Frontiers in microbiology · 2018Article
- Acid Stress Response Mechanisms of Group B Streptococci.Frontiers in cellular and infection microbiology · 2017Review
- Stress Physiology of Lactic Acid Bacteria.Microbiology and molecular biology reviews : MMBR · 2016Review
- Adaptive response of Group B streptococcus to high glucose conditions: new insights on the CovRS regulation network.PloS one · 2013Article
- Compatible solutes: the key to Listeria's success as a versatile gastrointestinal pathogen?Gut pathogens · 2010Article
- Compatible solutes: A listerial passe-partout?Gut microbes · 2010Article
- CsrRS regulates group B Streptococcus virulence gene expression in response to environmental pH: a new perspective on vaccine development.Journal of bacteriology · 2009Article
- HtrA is essential for efficient secretion of recombinant proteins by Lactococcus lactis.Applied and environmental microbiology · 2008Article
- The compatible-solute-binding protein OpuAC from Bacillus subtilis: ligand binding, site-directed mutagenesis, and crystallographic studies.Journal of bacteriology · 2008Article
- Pseudomonas syringae BetT is a low-affinity choline transporter that is responsible for superior osmoprotection by choline over glycine betaine.Journal of bacteriology · 2008Article
- Variation in the group B Streptococcus CsrRS regulon and effects on pathogenicity.Journal of bacteriology · 2008Article
- Defects in ex vivo and in vivo growth and sensitivity to osmotic stress of group A Streptococcus caused by interruption of response regulator gene vicR.Microbiology (Reading, England) · 2006Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cytoplasmic accumulation of exogenous betaine stimulates the growth of Lactococcus lactis cultivated under hyperosmotic conditions. We report that L. lactis possesses a single betaine transport system that belongs to the ATP-binding cassette (ABC) superfamily of transporters. Through transposon mutagenesis, a mutant deficient in betaine transport was isolated. We identified two genes, busAA and busAB, grouped in an operon, busA (betaine uptake system). The transcription of busA is strongly regulated by the external osmolality of the medium. The busAA gene codes for the ATP-binding protein. busAB encodes a 573-residue polypeptide which presents two striking features: (i) a fusion between the regions encoding the transmembrane domain (TMD) and the substrate-binding domain (SBD) and (ii) a swapping of the SBD subdomains when compared to the Bacillus subtilis betaine-binding protein, OpuAC. BusA of L. lactis displays a high affinity towards betaine (K(m) = 1.7 microM) and is an osmosensor whose activity is tightly regulated by external osmolality, leading the betaine uptake capacity of L. lactis to be under dual control at the biochemical and genetic levels. A protein presenting the characteristics predicted for BusAB was detected in the membrane fraction of L. lactis. The fusion between the TMD and the SBD is the first example of a new organization within prokaryotic ABC transporters.
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