ArticleMolecular biology of the cell1999
LC2, the chlamydomonas homologue of the t complex-encoded protein Tctex2, is essential for outer dynein arm assembly.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 63 citations in OpenAlex.
- Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii.PLoS genetics · 2024Article
- Review
- Light chain 2 is a Tctex-type related axonemal dynein light chain that regulates directional ciliary motility in Trypanosoma brucei.PLoS pathogens · 2022Article
- Ciliary Motility: Regulation of Axonemal Dynein Motors.Cold Spring Harbor perspectives in biology · 2017Review
- Evidence of positive selection associated with placental loss in tiger sharks.BMC evolutionary biology · 2016Article
- Structure and function of outer dynein arm intermediate and light chain complex.Molecular biology of the cell · 2016Article
- Subunit composition of the human cytoplasmic dynein-2 complex.Journal of cell science · 2014Article
- The awesome power of dikaryons for studying flagella and basal bodies in Chlamydomonas reinhardtii.Cytoskeleton (Hoboken, N.J.) · 2014Review
- TCTEX1D4, a novel protein phosphatase 1 interactor: connecting the phosphatase to the microtubule network.Biology open · 2013Article
- Integrated control of axonemal dynein AAA(+) motors.Journal of structural biology · 2012Review
- Synthesizing and salvaging NAD: lessons learned from Chlamydomonas reinhardtii.PLoS genetics · 2010Article
- Three members of the LC8/DYNLL family are required for outer arm dynein motor function.Molecular biology of the cell · 2008Article
- Dyneins across eukaryotes: a comparative genomic analysis.Traffic (Copenhagen, Denmark) · 2007Article
- Identification of predicted human outer dynein arm genes: candidates for primary ciliary dyskinesia genes.Journal of medical genetics · 2006Article
- Genetic analysis of the cytoplasmic dynein subunit families.PLoS genetics · 2006Article
- Differential light chain assembly influences outer arm dynein motor function.Molecular biology of the cell · 2005Article
- The LC7 light chains of Chlamydomonas flagellar dyneins interact with components required for both motor assembly and regulation.Molecular biology of the cell · 2004Article
- Oda5p, a novel axonemal protein required for assembly of the outer dynein arm and an associated adenylate kinase.Molecular biology of the cell · 2004Article
- DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC.Molecular biology of the cell · 2003Article
- Backbone dynamics of the 8 kDa dynein light chain dimer reveals molecular basis of the protein's functional diversity.Journal of biomolecular NMR · 2002Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Tctex2 is thought to be one of the distorter genes of the mouse t haplotype. This complex greatly biases the segregation of the chromosome that carries it such that in heterozygous +/t males, the t haplotype is transmitted to >95% of the offspring, a phenomenon known as transmission ratio distortion. The LC2 outer dynein arm light chain of Chlamydomonas reinhardtii is a homologue of the mouse protein Tctex2. We have identified Chlamydomonas insertional mutants with deletions in the gene encoding LC2 and demonstrate that the LC2 gene is the same as the ODA12 gene, the product of which had not been identified previously. Complete deletion of the LC2/ODA12 gene causes loss of all outer arms and a slow jerky swimming phenotype. Transformation of the deletion mutant with the cloned LC2/ODA12 gene restores the outer arms and rescues the motility phenotype. Therefore, LC2 is required for outer arm assembly. The fact that LC2 is an essential subunit of flagellar outer dynein arms allows us to propose a detailed mechanism whereby transmission ratio distortion is explained by the differential binding of mutant (t haplotype encoded) and wild-type dyneins to the axonemal microtubules of t-bearing or wild-type sperm, with resulting differences in their motility.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.