ArticleMolecular biology of the cell1999
Polarized sphingolipid transport from the subapical compartment: evidence for distinct sphingolipid domains.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 41 citations in OpenAlex.
- Analysis of polarized membrane traffic in hepatocytes and hepatic cell lines.Current protocols in cell biology · 2012Article
- Cholesterol regulation of rab-mediated sphingolipid endocytosis.Glycoconjugate journal · 2009Review
- Regulatory subunit I-controlled protein kinase A activity is required for apical bile canalicular lumen development in hepatocytes.The Journal of biological chemistry · 2009Article
- The chlamydial inclusion preferentially intercepts basolaterally directed sphingomyelin-containing exocytic vacuoles.Traffic (Copenhagen, Denmark) · 2008Article
- Protein kinase A-dependent step(s) in hepatitis C virus entry and infectivity.Journal of virology · 2008Article
- Anchoring of protein kinase A-regulatory subunit IIalpha to subapically positioned centrosomes mediates apical bile canalicular lumen development in response to oncostatin M but not cAMP.Molecular biology of the cell · 2007Article
- Efficient trafficking of MDR1/P-glycoprotein to apical canalicular plasma membranes in HepG2 cells requires PKA-RIIalpha anchoring and glucosylceramide.Molecular biology of the cell · 2006Article
- Polarized membrane traffic and cell polarity development is dependent on dihydroceramide synthase-regulated sphinganine turnover.Molecular biology of the cell · 2004Article
- Oncostatin M-stimulated apical plasma membrane biogenesis requires p27(Kip1)-regulated cell cycle dynamics.Molecular biology of the cell · 2004Article
- Raft-mediated trafficking of apical resident proteins occurs in both direct and transcytotic pathways in polarized hepatic cells: role of distinct lipid microdomains.Molecular biology of the cell · 2003Article
- Oncostatin M regulates membrane traffic and stimulates bile canalicular membrane biogenesis in HepG2 cells.The EMBO journal · 2002Article
- Different sphingolipids show differential partitioning into sphingolipid/cholesterol-rich domains in lipid bilayers.Biophysical journal · 2000Article
- Polarized sphingolipid transport from the subapical compartment changes during cell polarity development.Molecular biology of the cell · 2000Article
- Lipid membrane domains in cell surface and vacuolar systems.Glycoconjugate journalReview
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In polarized HepG2 cells, the sphingolipids glucosylceramide and sphingomyelin (SM), transported along the reverse transcytotic pathway, are sorted in subapical compartments (SACs), and subsequently targeted to either apical or basolateral plasma membrane domains, respectively. In the present study, evidence is provided that demonstrates that these sphingolipids constitute separate membrane domains at the luminal side of the SAC membrane. Furthermore, as revealed by the use of various modulators of membrane trafficking, such as calmodulin antagonists and dibutyryl-cAMP, it is shown that the fate of these separate sphingolipid domains is regulated by different signals, including those that govern cell polarity development. Thus under conditions that stimulate apical plasma membrane biogenesis, SM is rerouted from a SAC-to-basolateral to a SAC-to-apical pathway. The latter pathway represents the final leg in the transcytotic pathway, followed by the transcytotic pIgR-dIgA protein complex. Interestingly, this pathway is clearly different from the apical recycling pathway followed by glucosylceramide, further indicating that randomization of these pathways, which are both bound for the apical membrane, does not occur. The consequence of the potential coexistence of separate sphingolipid domains within the same compartment in terms of "raft" formation and apical targeting is discussed.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.