ArticleMolecular biology of the cell1999
A double-strand break repair component is essential for S phase completion in fission yeast cell cycling.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- DNA Repair in Haploid Context.International journal of molecular sciences · 2021Review
- Failed gene conversion leads to extensive end processing and chromosomal rearrangements in fission yeast.The EMBO journal · 2009Article
- Article
- Inhibition of BRCA1 in breast cell lines causes the centrosome duplication cycle to be disconnected from the cell cycle.Oncogene · 2006Article
- Multiple start codons and phosphorylation result in discrete Rad52 protein species.Nucleic acids research · 2006Article
- The F-Box DNA helicase Fbh1 prevents Rhp51-dependent recombination without mediator proteins.Molecular and cellular biology · 2005Article
- Article
- Histone H2A phosphorylation controls Crb2 recruitment at DNA breaks, maintains checkpoint arrest, and influences DNA repair in fission yeast.Molecular and cellular biology · 2004Article
- DNA repair by a Rad22-Mus81-dependent pathway that is independent of Rhp51.Nucleic acids research · 2004Article
- Schizosaccharomyces pombe Rdh54 (TID1) acts with Rhp54 (RAD54) to repair meiotic double-strand breaks.Molecular biology of the cell · 2003Article
- Pathway utilization in response to a site-specific DNA double-strand break in fission yeast.The EMBO journal · 2003Article
- Role of RAD52 epistasis group genes in homologous recombination and double-strand break repair.Microbiology and molecular biology reviews : MMBR · 2002Review
- A molecular genetic dissection of the evolutionarily conserved N terminus of yeast Rad52.Genetics · 2002Article
- Differential expression and requirements for Schizosaccharomyces pombe RAD52 homologs in DNA repair and recombination.Nucleic acids research · 2002Article
- Article
- The yeast recombinational repair protein Rad59 interacts with Rad52 and stimulates single-strand annealing.Genetics · 2001Article
- Homologous DNA recombination in vertebrate cells.Proceedings of the National Academy of Sciences of the United States of America · 2001Review
- Rad52 forms DNA repair and recombination centers during S phase.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Damage tolerance protein Mus81 associates with the FHA1 domain of checkpoint kinase Cds1.Molecular and cellular biology · 2000Article
- Fission yeast Eso1p is required for establishing sister chromatid cohesion during S phase.Molecular and cellular biology · 2000Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fission yeast rad22(+), a homologue of budding yeast RAD52, encodes a double-strand break repair component, which is dispensable for proliferation. We, however, have recently obtained a cell division cycle mutant with a temperature-sensitive allele of rad22(+), designated rad22-H6, which resulted from a point mutation in the conserved coding sequence leading to one amino acid alteration. We have subsequently isolated rad22(+) and its novel homologue rti1(+) as multicopy suppressors of this mutant. rti1(+) suppresses all the defects of cells lacking rad22(+). Mating type switch-inactive heterothallic cells lacking either rad22(+) or rti1(+) are viable, but those lacking both genes are inviable and arrest proliferation with a cell division cycle phenotype. At the nonpermissive temperature, a synchronous culture of rad22-H6 cells performs DNA synthesis without delay and arrests with chromosomes seemingly intact and replication completed and with a high level of tyrosine-phosphorylated Cdc2. However, rad22-H6 cells show a typical S phase arrest phenotype if combined with the rad1-1 checkpoint mutation. rad22(+) genetically interacts with rad11(+), which encodes the large subunit of replication protein A. Deletion of rad22(+)/rti1(+) or the presence of rad22-H6 mutation decreases the restriction temperature of rad11-A1 cells by 4-6 degrees C and leads to cell cycle arrest with chromosomes incompletely replicated. Thus, in fission yeast a double-strand break repair component is required for a certain step of chromosome replication unlinked to repair, partly via interacting with replication protein A.
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