ArticleMolecular biology of the cell1999
Rab5 induces Rac-independent lamellipodia formation and cell migration.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 86 citations in OpenAlex.
- A two-dimensional vertex model for curvy cell-cell interfaces at the subcellular scale.Journal of the Royal Society, Interface · 2024Article
- Article
- The Role of Akt/Rab5A Signalling in Regulating Cell Migration of MDA-MB-231 Breast Cancer Cell Line.Archives of Razi Institute · 2023Article
- Rab33b-exocyst interaction mediates localized secretion for focal adhesion turnover and cell migration.iScience · 2022Article
- Rab5 regulates the proliferation, migration and invasion of glioma cells via cyclin E.Oncology letters · 2020Article
- A NUMB-EFA6B-ARF6 recycling route controls apically restricted cell protrusions and mesenchymal motility.The Journal of cell biology · 2018Article
- Pro-migratory and TGF-β-activating functions of αvβ6 integrin in pancreatic cancer are differentially regulated via an Eps8-dependent GTPase switch.The Journal of pathology · 2017Article
- High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells.Journal of visualized experiments : JoVE · 2016Article
- The role of endocytic Rab GTPases in regulation of growth factor signaling and the migration and invasion of tumor cells.Small GTPases · 2015Review
- A novel caveolin-1/p85α/Rab5/Tiam1/Rac1 signaling axis in tumor cell migration and invasion.Communicative & integrative biology · 2014Article
- Phosphorylation of Rab5a protein by protein kinase Cϵ is crucial for T-cell migration.The Journal of biological chemistry · 2014Article
- Rab5 is required in metastatic cancer cells for Caveolin-1-enhanced Rac1 activation, migration and invasion.Journal of cell science · 2014Article
- Rab'ing tumor cell migration and invasion: focal adhesion disassembly driven by Rab5.Cell adhesion & migration · 2014Article
- Rab5 activation promotes focal adhesion disassembly, migration and invasiveness in tumor cells.Journal of cell science · 2013Article
- Klf5 controls bone marrow homing of stem cells and progenitors through Rab5-mediated β1/β2-integrin trafficking.Nature communications · 2013Article
- Caspase control: protagonists of cancer cell apoptosis.Experimental oncology · 2012Review
- Endocytic and recycling endosomes modulate cell shape changes and tissue behaviour during morphogenesis in Drosophila.PloS one · 2011Article
- Rab5 mediates caspase-8-promoted cell motility and metastasis.Molecular biology of the cell · 2010Article
- Rab5 isoforms differentially regulate the trafficking and degradation of epidermal growth factor receptors.The Journal of biological chemistry · 2009Article
- Caspase 8 promotes peripheral localization and activation of Rab5.The Journal of biological chemistry · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rab5 is a regulatory GTPase of vesicle docking and fusion that is involved in receptor-mediated endocytosis and pinocytosis. Introduction of active Rab5 in cells stimulates the rate of endocytosis and vesicle fusion, resulting in the formation of large endocytic vesicles, whereas dominant negative Rab5 inhibits vesicle fusion. Here we show that introduction of active Rab5 in fibroblasts also induced reorganization of the actin cytoskeleton but not of microtubule filaments, resulting in prominent lamellipodia formation. The Rab5-induced lamellipodia formation did not require activation of PI3-K or the GTPases Ras, Rac, Cdc42, or Rho, which are all strongly implicated in cytoskeletal reorganization. Furthermore, lamellipodia formation by insulin, Ras, or Rac was not affected by expression of dominant negative Rab5. In addition, cells expressing active Rab5 displayed a dramatic stimulation of cell migration, with the lamellipodia serving as the leading edge. Both lamellipodia formation and cell migration were dependent on actin polymerization but not on microtubules. These results demonstrate that Rab5 induces lamellipodia formation and cell migration and that the Rab5-induced lamellipodia formation occurs by a novel mechanism independent of, and distinct from, PI3-K, Ras, or Rho-family GTPases. Thus, Rab5 can control not only endocytosis but also actin cytoskeleton reorganization and cell migration, which provides strong support for an intricate relationship between these processes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.