ArticleMolecular biology of the cell1999
Complex patterns of alternative splicing mediate the spatial and temporal distribution of perlecan/UNC-52 in Caenorhabditis elegans.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 86 citations in OpenAlex.
- UNC-52 localization with respect to longitudinal axon tracts in the ventral and dorsal nerve cords inmicroPublication biology · 2025Article
- Laminin is uniformly expressed in basement membranes surrounding longitudinal axon tracts in the ventral and dorsal nerve cords inmicroPublication biology · 2025Article
- The RhoGAP RRC-1 is required for the assembly or stability of integrin adhesion complexes and is a member of the PIX pathway in muscle.Molecular biology of the cell · 2024Article
- Niche cells regulate primordial germ cell quiescence in response to basement membrane signaling.Development (Cambridge, England) · 2023Article
- Article
- Caenorhabditis elegans ETR-1/CELF has broad effects on the muscle cell transcriptome, including genes that regulate translation and neuroblast migration.BMC genomics · 2022Article
- GPN-1/glypican and UNC-52/perlecan do not appear to function in BMP signaling to pattern themicroPublication biology · 2021Article
- The Rho-GEF PIX-1 directs assembly or stability of lateral attachment structures between muscle cells.Nature communications · 2020Article
- Two alleles ofmicroPublication biology · 2020Article
- Deficit in the epidermal barrier induces toxicity and translocation of PEG modified graphene oxide in nematodes.Toxicology research · 2018Article
- Four specific immunoglobulin domains in UNC-52/Perlecan function with NID-1/Nidogen during dendrite morphogenesis inDevelopment (Cambridge, England) · 2018Article
- Genetic Suppression of Basement Membrane Defects inGenetics · 2018Article
- High-resolution imaging of muscle attachment structures in Caenorhabditis elegans.Cytoskeleton (Hoboken, N.J.) · 2017Article
- A current view of perlecan in physiology and pathology: A mosaic of functions.Matrix biology : journal of the International Society for Matrix Biology · 2017Review
- Functional Requirements for Heparan Sulfate Biosynthesis in Morphogenesis and Nervous System Development in C. elegans.PLoS genetics · 2017Article
- Sarcomeres Pattern Proprioceptive Sensory Dendritic Endings through UNC-52/Perlecan in C. elegans.Developmental cell · 2015Article
- An active role for basement membrane assembly and modification in tissue sculpting.Journal of cell science · 2015Review
- Conditional control of alternative splicing through light-triggered splice-switching oligonucleotides.Journal of the American Chemical Society · 2015Article
- Basement Membranes in the Worm: A Dynamic Scaffolding that Instructs Cellular Behaviors and Shapes Tissues.Current topics in membranes · 2015Review
- Syndecan defines precise spindle orientation by modulating Wnt signaling in C. elegans.Development (Cambridge, England) · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The unc-52 gene encodes the nematode homologue of mammalian perlecan, the major heparan sulfate proteoglycan of the extracellular matrix. This is a large complex protein with regions similar to low-density lipoprotein receptors, laminin, and neural cell adhesion molecules (NCAMs). In this study, we extend our earlier work and demonstrate that a number of complex isoforms of this protein are expressed through alternative splicing. We identified three major classes of perlecan isoforms: a short form lacking the NCAM region and the C-terminal agrin-like region; a medium form containing the NCAM region, but still lacking the agrin-like region; and a newly identified long form that contains all five domains present in mammalian perlecan. Using region-specific antibodies and unc-52 mutants, we reveal a complex spatial and temporal expression pattern for these UNC-52 isoforms. As well, using a series of mutations affecting different regions and thus different isoforms of UNC-52, we demonstrate that the medium NCAM-containing isoforms are sufficient for myofilament lattice assembly in developing nematode body-wall muscle. Neither short isoforms nor isoforms containing the C-terminal agrin-like region are essential for sarcomere assembly or muscle cell attachment, and their role in development remains unclear.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.