ArticleMolecular biology of the cell1999
Caldesmon inhibits nonmuscle cell contractility and interferes with the formation of focal adhesions.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
62 citing papers in PubMed, 196 citations in OpenAlex.
- Advances in mechanotransduction and sonobiology: effects of audible acoustic waves and low-vibration stimulations on mammalian cells.Biophysical reviews · 2024Review
- Focal adhesions are controlled by microtubules through local contractility regulation.The EMBO journal · 2024Article
- Glucocorticoid receptor-induced non-muscle caldesmon regulates metastasis in castration-resistant prostate cancer.Oncogenesis · 2023Article
- Static and Dynamic: Evolving Biomaterial Mechanical Properties to Control Cellular Mechanotransduction.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Caldesmon controls stress fiber force-balance through dynamic cross-linking of myosin II and actin-tropomyosin filaments.Nature communications · 2022Article
- Emerging role of caldesmon in cancer: A potential biomarker for colorectal cancer and other cancers.World journal of gastrointestinal oncology · 2022Review
- Subcellular Force Quantification of Endothelial Cells Using Silicone Pillar Arrays.Methods in molecular biology (Clifton, N.J.) · 2022Article
- CALD1 promotes the expression of PD-L1 in bladder cancer via the JAK/STAT signaling pathway.Annals of translational medicine · 2021Article
- (De)form and Function: Measuring Cellular Forces with Deformable Materials and Deformable Structures.Advanced healthcare materials · 2020Review
- Micro- and nanodevices integrated with biomolecular probes.Biotechnology advances · 2015Review
- Integration of actin dynamics and cell adhesion by a three-dimensional, mechanosensitive molecular clutch.Nature cell biology · 2015Review
- β-PIX controls intracellular viscoelasticity to regulate lung cancer cell migration.Journal of cellular and molecular medicine · 2015Article
- Investigating cell mechanics with atomic force microscopy.Journal of the Royal Society, Interface · 2015Article
- Intrinsically disordered caldesmon binds calmodulin via the "buttons on a string" mechanism.PeerJ · 2015Article
- Modulation of Epidermal Growth Factor Stimulated ERK Phosphorylation and Cell Motility by Inositol Trisphosphate Kinase.Journal of pharmaceutical sciences and pharmacology · 2014Article
- New insight on the molecular aspects of glucocorticoid effects in nervous system development.Journal of endocrinological investigation · 2013Review
- Mechanotransduction at focal adhesions: integrating cytoskeletal mechanics in migrating cells.Journal of cellular and molecular medicine · 2013Review
- Article
- Up-regulated expression of l-caldesmon associated with malignancy of colorectal cancer.BMC cancer · 2012Article
- The conformational state of actin filaments regulates branching by actin-related protein 2/3 (Arp2/3) complex.The Journal of biological chemistry · 2012Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
Caldesmon is known to inhibit the ATPase activity of actomyosin in a Ca(2+)-calmodulin-regulated manner. Although a nonmuscle isoform of caldesmon is widely expressed, its functional role has not yet been elucidated. We studied the effects of nonmuscle caldesmon on cellular contractility, actin cytoskeletal organization, and the formation of focal adhesions in fibroblasts. Transient transfection of nonmuscle caldesmon prevents myosin II-dependent cell contractility and induces a decrease in the number and size of tyrosine-phosphorylated focal adhesions. Expression of caldesmon interferes with Rho A-V14-mediated formation of focal adhesions and stress fibers as well as with formation of focal adhesions induced by microtubule disruption. This inhibitory effect depends on the actin- and myosin-binding regions of caldesmon, because a truncated variant lacking both of these regions is inactive. The effects of caldesmon are blocked by the ionophore A23187, thapsigargin, and membrane depolarization, presumably because of the ability of Ca(2+)-calmodulin or Ca(2+)-S100 proteins to antagonize the inhibitory function of caldesmon on actomyosin contraction. These results indicate a role for nonmuscle caldesmon in the physiological regulation of actomyosin contractility and adhesion-dependent signaling and further demonstrate the involvement of contractility in focal adhesion formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.