ArticleBiophysical journal1999
Cooperative block of the plant endomembrane ion channel by ruthenium red.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 37 citations in OpenAlex.
- Mechanism of calcium signal response to cadmium stress in duckweed.Plant signaling & behavior · 2022Article
- Nano-enabled agriculture: How do nanoparticles cross barriers in plants?Plant communications · 2022Review
- Review
- Pharmacological Strategies for Manipulating Plant CaInternational journal of molecular sciences · 2018Review
- Vacuolar ion channels in the liverwort Marchantia polymorpha: influence of ion channel inhibitors.Planta · 2017Article
- Dissecting blue light signal transduction pathway in leaf epidermis using a pharmacological approach.Planta · 2015Article
- Biological physics in México: Review and new challenges.Journal of biological physics · 2011Article
- Plant volatiles regulate the activities of Ca2+ -permeable channels and promote cytoplasmic calcium transients in Arabidopsis leaf cells.Plant signaling & behavior · 2009Article
- Article
- Cytoplasmic calcium increases in response to changes in the gravity vector in hypocotyls and petioles of Arabidopsis seedlings.Plant physiology · 2008Article
- Discovery of glycine hydrazide pore-occluding CFTR inhibitors: mechanism, structure-activity analysis, and in vivo efficacy.The Journal of general physiology · 2004Article
- The gating kinetics of the slow vacuolar channel. A novel mechanism for SV channel functioning?The Journal of membrane biology · 2003Article
- Nickel inhibits the slowly activating channels of radish vacuoles.European biophysics journal : EBJ · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Effects of ruthenium red (RR) on the slow Ca(2+)-activated Ca(2+)-permeable vacuolar channel have been studied by patch-clamp technique. Applied to the cytosolic side of isolated membrane patches, RR at concentrations of 0.1-5 microM produced two distinct effects on single channel kinetics, long lasting closures and a flickering block of the open state. The first effect was largely irreversible, whereas the second one could be washed out. The extent of flickering block steeply increased (zdelta = approximately 1.35) with the increase of cytosol-positive voltage, dragging RR into the channel pore. At least two RR ions are involved in the block according to Hill coefficient n = approximately 1.30 for the dose response curves. The on-rate rate of the drug binding linearly depended on the RR concentration, implying that one RR ion already plugged the pore. The blocked state was further stabilized by binding of the second RR. This stabilization was in excess of that predicted by independent binding as the dependence of unblocking rate on RR concentration revealed. A cooperative model was therefore employed to describe the kinetic behavior of RR binding. At zero voltage the half-blocking RR concentration of 36 microM and the bimolecular on-rate constant of 1.8 x 10(8) M(-1) s(-1) were estimated.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.