ArticleThe Journal of clinical investigation1999
Heparin-binding protein targeted to mitochondrial compartments protects endothelial cells from apoptosis.
Article in The Journal of clinical investigation, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 61 citations in OpenAlex.
- The effect of heparins on plasma concentration of heparin-binding protein: a pilot study.BJA open · 2024Article
- The Dynamics of Circulating Heparin-Binding Protein: Implications for Its Use as a Biomarker.Journal of innate immunity · 2022Article
- A Promising Candidate: Heparin-Binding Protein Steps onto the Stage of Sepsis Prediction.Journal of immunology research · 2019Review
- Article
- Heparin-binding protein is important for vascular leak in sepsis.Intensive care medicine experimental · 2016Article
- Neutrophil-Derived Proteases in the Microenvironment of Pancreatic Cancer -Active Players in Tumor Progression.International journal of biological sciences · 2016Review
- Azurocidin-induced inhibition of oxygen metabolism in mitochondria is antagonized by heparin.Experimental and therapeutic medicine · 2014Article
- Opening the flood-gates: how neutrophil-endothelial interactions regulate permeability.Trends in immunology · 2009Review
- Neutrophil secretion products regulate anti-bacterial activity in monocytes and macrophages.Clinical and experimental immunology · 2008Article
- Glypican-3 is involved in cellular protection against mitoxantrone in gastric carcinoma cells.Oncogene · 2004Article
- CAP37, a novel inflammatory mediator: its expression in endothelial cells and localization to atherosclerotic lesions.The American journal of pathology · 2002Article
- Internalization of proteinase 3 is concomitant with endothelial cell apoptosis and internalization of myeloperoxidase with generation of intracellular oxidants.The American journal of pathology · 2001Article
- Paracrine control of endothelial cell survival.The Journal of clinical investigation · 1999Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neutrophil-borne heparin-binding protein (HBP) is a multifunctional protein involved in the progression of inflammation. HBP is stored in neutrophil granules and released upon stimulation of the cells in proximity to endothelial cells. HBP affects endothelial cells in multiple ways; however, the molecular and cellular mechanisms underlying the interaction of HBP with these cells are unknown. Affinity isolation and enzymatic degradation demonstrated that HBP released from human neutrophils binds to endothelial cell-surface proteoglycans, such as syndecans and glypican. Flow cytometry indicated that a significant fraction of proteoglycan-bound HBP is taken up by the endothelial cells, and we used radiolabeled HBP to determine the internalization rate of surface-bound HBP. Confocal and electron microscopy revealed that internalized HBP is targeted to perinuclear compartments of endothelial cells, where it colocalizes with mitochondria. Western blotting of isolated mitochondria from HBP-treated endothelial cells showed that HBP is present in 2 forms - 28 and 22 kDa. Internalized HBP markedly reduced growth factor deprivation-induced caspase-3 activation and protected endothelial cells from apoptosis, suggesting that uptake and intracellular routing of exogenous HBP to mitochondria contributes to the sustained viability of endothelial cells in the context of locally activated neutrophils.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.