ArticleThe Journal of cell biology1999
Plasma membrane microdomains act as concentration platforms to facilitate intoxication by aerolysin.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
65 citing papers in PubMed, 155 citations in OpenAlex.
- ThemBio · 2025Article
- The structural features and immunological role of biomphalysins in the snail Biomphalaria glabrata.PLoS pathogens · 2025Article
- Probing Bacterial Interactions with theToxins · 2025Article
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- High-affinity biomolecular interactions are modulated by low-affinity binders.NPJ systems biology and applications · 2024Article
- TurboID screening of ApxI toxin interactants identifies host proteins involved in Actinobacillus pleuropneumoniae-induced apoptosis of immortalized porcine alveolar macrophages.Veterinary research · 2023Article
- Involvement of Lipid Rafts in the Invasion of Opportunistic BacteriaInternational journal of molecular sciences · 2023Article
- Measuring Membrane Penetration Depths and Conformational Changes in Membrane Peptides and Proteins.The Journal of membrane biology · 2022Review
- Lipid larceny: channelizing host lipids for establishing successful pathogenesis by bacteria.Virulence · 2021Review
- Vaginal Isolates of Candida glabrata Are Uniquely Susceptible to Ionophoric Killer Toxins Produced by Saccharomyces cerevisiae.Antimicrobial agents and chemotherapy · 2021Article
- Article
- Mammalian membrane trafficking as seen through the lens of bacterial toxins.Cellular microbiology · 2020Review
- Article
- Anthrax toxin requires ZDHHC5-mediated palmitoylation of its surface-processing host enzymes.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Pneumolysin-damaged cells benefit from non-homogeneous toxin binding to cholesterol-rich membrane domains.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2018Article
- Morin Protects Channel Catfish FromFrontiers in microbiology · 2018Article
- Roles of the Mevalonate Pathway and Cholesterol Trafficking in Pulmonary Host Defense.Current molecular pharmacology · 2017Article
- GsdmD p30 elicited by caspase-11 during pyroptosis forms pores in membranes.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Revealing Assembly of a Pore-Forming Complex Using Single-Cell Kinetic Analysis and Modeling.Biophysical journal · 2016Article
- Structural basis for receptor recognition and pore formation of a zebrafish aerolysin-like protein.EMBO reports · 2016Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has been proposed that the plasma membrane of many cell types contains cholesterol-sphingolipid-rich microdomains. Here, we analyze the role of these microdomains in promoting oligomerization of the bacterial pore-forming toxin aerolysin. Aerolysin binds to cells, via glycosyl phosphatidylinositol-anchored receptors, as a hydrophilic soluble protein that must polymerize into an amphipathic ring-like complex to form a pore. We first show that oligomerization can occur at >10(5)-fold lower toxin concentration at the surface of living cells than in solution. Our observations indicate that it is not merely the number of receptors on the target cell that is important for toxin sensitivity, but their ability to associate transiently with detergent resistant microdomains. Oligomerization appears to be promoted by the fact that the toxin bound to its glycosyl phosphatidylinositol-anchored receptors, can be recruited into these microdomains, which act as concentration devices.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.