ArticleThe Journal of cell biology1999
Cytoplasmic dynein is required for distinct aspects of MTOC positioning, including centrosome separation, in the one cell stage Caenorhabditis elegans embryo.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 218 papers.
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218 citing papers in PubMed, 451 citations in OpenAlex.
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- Worm orthologues of cytokinesis-associated proteins CIT and ASPM regulate neuronal microtubule dynamics and polarity in C. elegans.PLoS genetics · 2026Article
- Multiscale temporal tuning of force generation complex machinery governs cortical microtubule interactions during the first mitotic division inbioRxiv : the preprint server for biology · 2026Article
- The KASH protein UNC-83 differentially regulates kinesin-1 activity to control developmental stage-specific nuclear migration.Current biology : CB · 2025Article
- Hybrid incompatibility emerges at the one-cell stage in interspecies Caenorhabditis embryos.Current biology : CB · 2025Article
- Hybrid incompatibility emerges at the one-cell stage in interspeciesbioRxiv : the preprint server for biology · 2025Article
- Asymmetry in centrosome maturation revealed through AIR-1 dynamics in the early Caenorhabditis elegans embryo.Scientific reports · 2025Article
- Live-cell imaging under centrifugation characterized the cellular force for nuclear centration in theProceedings of the National Academy of Sciences of the United States of America · 2024Article
- ThebioRxiv : the preprint server for biology · 2024Article
- Specific kinesin and dynein molecules participate in the unconventional protein secretion of transmembrane proteins.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2024Article
- The LINC complex ensures accurate centrosome positioning during prophase.Life science alliance · 2024Article
- Dynein directs prophase centrosome migration to control the stem cell division axis in the developing Caenorhabditis elegans epidermis.Genetics · 2024Article
- Live-cell imaging under centrifugation characterized the cellular force for nuclear centration in thebioRxiv : the preprint server for biology · 2024Article
- Enucleation of theLife science alliance · 2024Article
- Model-based trajectory classification of anchored molecular motor-biopolymer interactions.Biophysical reports · 2023Article
- Centriole elimination during Caenorhabditis elegans oogenesis initiates with loss of the central tube protein SAS-1.The EMBO journal · 2023Article
- Centrosome linker diversity and its function in centrosome clustering and mitotic spindle formation.The EMBO journal · 2023Article
- An Emerging Animal Model for Querying the Role of Whole Genome Duplication in Development, Evolution, and Disease.Journal of developmental biology · 2023Review
- The meiotic LINC complex component KASH5 is an activating adaptor for cytoplasmic dynein.The Journal of cell biology · 2023Article
158 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated the role of cytoplasmic dynein in microtubule organizing center (MTOC) positioning using RNA-mediated interference (RNAi) in Caenorhabditis elegans to deplete the product of the dynein heavy chain gene dhc-1. Analysis with time-lapse differential interference contrast microscopy and indirect immunofluorescence revealed that pronuclear migration and centrosome separation failed in one cell stage dhc-1 (RNAi) embryos. These phenotypes were also observed when the dynactin components p50/dynamitin or p150(Glued) were depleted with RNAi. Moreover, in 15% of dhc-1 (RNAi) embryos, centrosomes failed to remain in proximity of the male pronucleus. When dynein heavy chain function was diminished only partially with RNAi, centrosome separation took place, but orientation of the mitotic spindle was defective. Therefore, cytoplasmic dynein is required for multiple aspects of MTOC positioning in the one cell stage C. elegans embryo. In conjunction with our observation of cytoplasmic dynein distribution at the periphery of nuclei, these results lead us to propose a mechanism in which cytoplasmic dynein anchored on the nucleus drives centrosome separation.
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