ArticleThe Journal of cell biology1999
Spatial relationship between transcription sites and chromosome territories.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
82 citing papers in PubMed, 251 citations in OpenAlex.
- Chromatin unfolding via loops can drive clustered transposon insertion.Biophysical journal · 2025Article
- Spatial promoter-enhancer hubs in cancer: organization, regulation, and function.Trends in cancer · 2023Review
- Mini-review: Gene regulatory network benefits from three-dimensional chromatin conformation and structural biology.Computational and structural biotechnology journal · 2023Review
- Spatiotemporal Epigenetic Control of the Histone Gene Chromatin Landscape during the Cell Cycle.Critical reviews in eukaryotic gene expression · 2023Article
- Simulation of Different Three-Dimensional Models of Whole Interphase Nuclei Compared to Experiments - A Consistent Scale-Bridging Simulation Framework for Genome Organization.Results and problems in cell differentiation · 2022Article
- Epigenetic-Mediated Regulation of Gene Expression for Biological Control and Cancer: Cell and Tissue Structure, Function, and Phenotype.Results and problems in cell differentiation · 2022Review
- Plant 3D Chromatin Organization: Important Insights from Chromosome Conformation Capture Analyses of the Last 10 Years.Plant & cell physiology · 2021Review
- Transcription organizes euchromatin via microphase separation.Nature communications · 2021Article
- Spatial Organization of Chromatin: Transcriptional Control of Adaptive Immune Cell Development.Frontiers in immunology · 2021Review
- Methods for mapping 3D chromosome architecture.Nature reviews. Genetics · 2020Review
- Higher order genomic organization and epigenetic control maintain cellular identity and prevent breast cancer.Genes, chromosomes & cancer · 2019Review
- Article
- The Role of RNA Polymerase II Contiguity and Long-Range Interactions in the Regulation of Gene Expression in Human Pluripotent Stem Cells.Stem cells international · 2019Article
- Chromosome positioning and male infertility: it comes with the territory.Journal of assisted reproduction and genetics · 2018Review
- Formation of correlated chromatin domains at nanoscale dynamic resolution during transcription.Nucleic acids research · 2018Article
- Quantitative imaging of mammalian transcriptional dynamics: from single cells to whole embryos.BMC biology · 2016Review
- Article
- Morphology of nuclear transcription.Histochemistry and cell biology · 2016Review
- Dynamic properties of independent chromatin domains measured by correlation spectroscopy in living cells.Epigenetics & chromatin · 2016Article
- The detailed 3D multi-loop aggregate/rosette chromatin architecture and functional dynamic organization of the human and mouse genomes.Epigenetics & chromatin · 2016Article
22 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated the spatial relationship between transcription sites and chromosome territories in the interphase nucleus of human female fibroblasts. Immunolabeling of nascent RNA was combined with visualization of chromosome territories by fluorescent in situ hybridization (FISH). Transcription sites were found scattered throughout the territory of one of the two X chromosomes, most likely the active X chromosome, and that of both territories of chromosome 19. The other X chromosome territory, probably the inactive X chromosome, was devoid of transcription sites. A distinct substructure was observed in interphase chromosome territories. Intensely labeled subchromosomal domains are surrounded by less strongly labeled areas. The intensely labeled domains had a diameter in the range of 300-450 nm and were sometimes interconnected, forming thread-like structures. Similar large scale chromatin structures were observed in HeLa cells expressing green fluorescent protein (GFP)-tagged histone H2B. Strikingly, nascent RNA was almost exclusively found in the interchromatin areas in chromosome territories and in between strongly GFP-labeled chromatin domains. These observations support a model in which transcriptionally active chromatin in chromosome territories is markedly compartmentalized. Active loci are located predominantly at or near the surface of compact chromatin domains, depositing newly synthesized RNA directly into the interchromatin space.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.