ArticleThe Journal of cell biology1999
ADP-ribosylation factor 6 and endocytosis at the apical surface of Madin-Darby canine kidney cells.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 147 citations in OpenAlex.
- Iron triggersmBio · 2025Article
- Roles for ELMOD2 and Rootletin in ciliogenesis.Molecular biology of the cell · 2021Article
- Novel function for AP-1B during cell migration.Molecular biology of the cell · 2020Article
- The small GTPase ARF-1.2 is a regulator of unicellular tube formation in Caenorhabditis elegans.The journal of physiological sciences : JPS · 2019Article
- Insane in the apical membrane: Trafficking events mediating apicobasal epithelial polarity during tube morphogenesis.Traffic (Copenhagen, Denmark) · 2018Review
- ELMOD1 Stimulates ARF6-GTP Hydrolysis to Stabilize Apical Structures in Developing Vestibular Hair Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Cellular and subcellular localization of ADP-ribosylation factor 6 in mouse peripheral tissues.Histochemistry and cell biology · 2017Article
- Rab14 specifies the apical membrane through Arf6-mediated regulation of lipid domains and Cdc42.Scientific reports · 2016Article
- PERP, a host tetraspanning membrane protein, is required for Salmonella-induced inflammation.Cellular microbiology · 2015Article
- Arf6 exchange factor EFA6 and endophilin directly interact at the plasma membrane to control clathrin-mediated endocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Polar delivery in plants; commonalities and differences to animal epithelial cells.Open biology · 2014Review
- Arf6 regulation of Gyrating-clathrin.Traffic (Copenhagen, Denmark) · 2013Article
- Toxicity mechanisms of amphotericin B and its neutralization by conjugation with arabinogalactan.Antimicrobial agents and chemotherapy · 2012Article
- Arf6 regulates AP-1B-dependent sorting in polarized epithelial cells.The Journal of cell biology · 2011Article
- Actin dynamics counteract membrane tension during clathrin-mediated endocytosis.Nature cell biology · 2011Article
- Fission yeast ucp3 gene encodes a putative Arf6 GTPase-activating protein.Molecular biology reports · 2011Article
- Bardet-Biedl syndrome-associated small GTPase ARL6 (BBS3) functions at or near the ciliary gate and modulates Wnt signaling.The Journal of biological chemistry · 2010Article
- alpha-Synuclein abnormalities in mouse models of peroxisome biogenesis disorders.Journal of neuroscience research · 2010Article
- Potassium channel silencing by constitutive endocytosis and intracellular sequestration.The Journal of biological chemistry · 2010Article
- Dynamin participates in the maintenance of anterior polarity in the Caenorhabditis elegans embryo.Developmental cell · 2009Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
We report that the small GTPase, ADP-ribosylation factor 6 (ARF6), is present only on the apical surface of polarized MDCK epithelial cells. Overexpression of a mutant of ARF6, ARF6-Q67L, which is predicted to be in the GTP-bound form, stimulates endocytosis exclusively at this surface. Surprisingly, overexpression of the mutant ARF6-T27N, which is predicted to be in the GDP-bound form, also stimulated apical endocytosis, though to a lesser extent. ARF6-stimulated endocytosis is inhibited by a dominant-negative form of dynamin, or a dominant-negative hub fragment of clathrin heavy chain, indicating that it is mediated by clathrin. Correspondingly, overexpression of either mutant of ARF6 leads to an increase in the number of clathrin-coated pits at the apical plasma membrane. When ARF6-Q67L is overexpressed in the presence of the dominant-negative dynamin, the ARF6-Q67L colocalizes with clathrin and with IgA bound to its receptor. We conclude that ARF6 is an important modulator of clathrin-mediated endocytosis at the apical surface of epithelial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.