ArticleApplied and environmental microbiology1999
Detection and differentiation of Listeria spp. by a single reaction based on multiplex PCR.
Article in Applied and environmental microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed.
- Molecular Characterization ofAnimals : an open access journal from MDPI · 2026Article
- Genomic and pathogenic characterization of Listeria ivanovii subsp. londoniensis from ovine farms in China.BMC veterinary research · 2026Article
- Nano-coating with silicon dioxide to reduce the occurrence of bacterial contamination in a pig abattoir drinking system.Folia microbiologica · 2025Article
- Antimicrobial resistance and prevalence of Listeria species from raw milk and dairy products in Burdur, Turkey.Veterinary medicine and science · 2024Article
- Molecular detection of bacterial zoonotic abortive agents from ruminants in Turkey.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- AFB1 induced free radicals cause encephalopathy in goat kids via intrinsic pathway of apoptosis: pathological and immunohistochemical confirmation of non-hepatic neuroaflatoxicosis.Veterinary research communications · 2024Article
- Exploring the occurrence ofFrontiers in microbiology · 2024Article
- Drinking Pipes and Nipple Drinkers in Pig Abattoir Lairage Pens-A Source of Zoonotic Pathogens as a Hazard to Meat Safety.Microorganisms · 2023Article
- A review of the literature ofFrontiers in microbiology · 2023Review
- Microbial Indicators and Possible Focal Points of Contamination during Production and Processing of Catfish.Foods (Basel, Switzerland) · 2022Article
- Novel Approaches to Environmental Monitoring and Control ofFoods (Basel, Switzerland) · 2022Review
- Identification and serotyping ofItalian journal of food safety · 2021Article
- Monitoring by a Sensitive Liquid-Based Sampling Strategy Reveals a Considerable Reduction ofFoods (Basel, Switzerland) · 2021Article
- Microbiological Safety and Sensory Quality of Cultivated Mushrooms (Foods (Basel, Switzerland) · 2021Article
- Prevalence and Genetic Diversity ofFrontiers in microbiology · 2021Article
- Co-Occurrence ofFrontiers in microbiology · 2021Article
- Draft Genome Sequence of Listeria monocytogenes Serovar 1/2a Strain IZSAM_Lm_14-16064, Isolated from an Italian Cooked Ham in 2014.Microbiology resource announcements · 2020Article
- Large Nationwide Outbreak of Invasive Listeriosis Associated with Blood Sausage, Germany, 2018-2019.Emerging infectious diseases · 2020Article
- Genomic-based identification of environmental and clinical Listeria monocytogenes strains associated with an abortion outbreak in beef heifers.BMC veterinary research · 2020Article
- Predominance of DistinctMicroorganisms · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The iap gene encodes the protein p60, which is common to all Listeria species. A previous comparison of the DNA sequences indicated conserved and species-specific gene portions. Based on these comparisons, a combination consisting of only five different primers that allows the specific detection and differentiation of Listeria species with a single multiplex PCR and subsequent gel analysis was selected. One primer was derived from the conserved 3' end and is specific for all Listeria species; the other four primers are specific for Listeria monocytogenes, L. innocua, L. grayi, or the three grouped species L. ivanovii, L. seeligeri, and L. welshimeri, respectively. The PCR method, which also enables the simultaneous detection of L. monocytogenes and L. innocua, was evaluated against conventional biotyping with 200 food hygiene-relevant Listeria strains. The results indicated the superiority of this technique. Thus, this novel type of multiplex PCR may be useful for rapid Listeria species confirmation and for identification of Listeria species for strains isolated from different sources.
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