ArticleApplied and environmental microbiology1999
Utilization of trihalogenated propanes by Agrobacterium radiobacter AD1 through heterologous expression of the haloalkane dehalogenase from Rhodococcus sp. strain M15-3.
Article in Applied and environmental microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 50 citations in OpenAlex.
- A Haloalkane Dehalogenase DhaA Nanoparticle Based on Pullulan Conjugation and Polyethyleneimine Adsorption.Applied biochemistry and biotechnology · 2025Article
- Engineering Enzymes for Environmental Sustainability.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2023Review
- Engineering Enzymes for Environmental Sustainability.Angewandte Chemie (International ed. in English) · 2023Review
- Bisphenol A-A Dangerous Pollutant Distorting the Biological Properties of Soil.International journal of molecular sciences · 2021Article
- A Pseudomonas putida strain genetically engineered for 1,2,3-trichloropropane bioremediation.Applied and environmental microbiology · 2014Article
- Novel dehalogenase mechanism for 2,3-dichloro-1-propanol utilization in Pseudomonas putida strain MC4.Applied and environmental microbiology · 2012Article
- Transformation and biodegradation of 1,2,3-trichloropropane (TCP).Environmental science and pollution research international · 2012Review
- Development of a dehalogenase-based protein fusion tag capable of rapid, selective and covalent attachment to customizable ligands.Current chemical genomics · 2012Article
- Crystallization and crystallographic analysis of the Rhodococcus rhodochrous NCIMB 13064 DhaA mutant DhaA31 and its complex with 1,2,3-trichloropropane.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011Article
- Redesigning dehalogenase access tunnels as a strategy for degrading an anthropogenic substrate.Nature chemical biology · 2009Article
- Biochemical characterization of haloalkane dehalogenases DrbA and DmbC, Representatives of a Novel Subfamily.Applied and environmental microbiology · 2009Article
- Application of nitroarene dioxygenases in the design of novel strains that degrade chloronitrobenzenes.Microbial biotechnology · 2009Article
- Crystals of DhaA mutants from Rhodococcus rhodochrous NCIMB 13064 diffracted to ultrahigh resolution: crystallization and preliminary diffraction analysis.Acta crystallographica. Section F, Structural biology and crystallization communications · 2008Article
- Cloning and expression of the haloalkane dehalogenase gene dhmA from Mycobacterium avium N85 and preliminary characterization of DhmA.Applied and environmental microbiology · 2002Article
- Biodegradation of 1,2,3-trichloropropane through directed evolution and heterologous expression of a haloalkane dehalogenase gene.Applied and environmental microbiology · 2002Article
- Halohydrin dehalogenases are structurally and mechanistically related to short-chain dehydrogenases/reductases.Journal of bacteriology · 2001Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trihalogenated propanes are toxic and recalcitrant organic compounds. Attempts to obtain pure bacterial cultures able to use these compounds as sole carbon and energy sources were unsuccessful. Both the haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 (DhlA) and that from Rhodococcus sp. strain m15-3 (DhaA) were found to dehalogenate trihalopropanes to 2,3-dihalogenated propanols, but the kinetic properties of the latter enzyme are much better. Broad-host-range dehalogenase expression plasmids, based on RSF1010 derivatives, were constructed with the haloalkane dehalogenase from Rhodococcus sp. strain m15-3 under the control of the heterologous promoters P(lac), P(dhlA), and P(trc). The resulting plasmids yielded functional expression in several gram-negative bacteria. A catabolic pathway for trihalopropanes was designed by introducing these broad-host-range dehalogenase expression plasmids into Agrobacterium radiobacter AD1, which has the ability to utilize dihalogenated propanols for growth. The recombinant strain AD1(pTB3), expressing the haloalkane dehalogenase gene under the control of the dhlA promoter, was able to utilize both 1,2,3-tribromopropane and 1,2-dibromo-3-chloropropane as sole carbon sources. Moreover, increased expression of the haloalkane dehalogenase resulted in elevated resistance to trihalopropanes.
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