Evidence map›Paper›PMID 10508057›Full record

ArticleApplied and environmental microbiology1999

Two cellobiohydrolase-encoding genes from Aspergillus niger require D-xylose and the xylanolytic transcriptional activator XlnR for their expression.

M M Gielkens, E Dekkers, J Visser, L H de Graaff

Open access · bronzeAbstract read
In one paragraph

Article in Applied and environmental microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.

0numbers the graph read from it
0cells of the map it votes in
70citing papers in PubMed
6.2field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

70 citing papers in PubMed, 206 citations in OpenAlex.

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  11. The regulatory and transcriptional landscape associated with carbon utilization in a filamentous fungus.Proceedings of the National Academy of Sciences of the United States of America · 2020
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  19. Biotechnology for biofuels · 2019
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  20. Article

10 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

M M GielkensSection Molecular Genetics of Industrial Microorganisms, Wageningen Agricultural University, NL-6703 HA Wageningen, The Netherlands.
E Dekkers
J Visser
L H de Graaff

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Two cellobiohydrolase-encoding genes, cbhA and cbhB, have been isolated from the filamentous fungus Aspergillus niger. The deduced amino acid sequence shows that CbhB has a modular structure consisting of a fungus-type cellulose-binding domain (CBD) and a catalytic domain separated by a Pro/Ser/Thr-rich linker peptide. CbhA consists only of a catalytic domain and lacks a CBD and linker peptide. Both proteins are homologous to fungal cellobiohydrolases in family 7 of the glycosyl hydrolases. Northern blot analysis showed that the transcription of the cbhA and cbhB genes is induced by D-xylose but not by sophorose and, in addition, requires the xylanolytic transcriptional activator XlnR.

Indexed as

Fungal ProteinsGenes, FungalAmino Acid SequenceAspergillus nigerBinding SitesCellulaseCelluloseCellulose 1,4-beta-CellobiosidaseCloning, MolecularMolecular Sequence DataSequence AlignmentTrans-ActivatorsXyloseCellulaseCelluloseCellulose 1,4-beta-CellobiosidaseFungal ProteinsTrans-ActivatorsXlnR protein, AspergillusXylose

Identifiers

PMID10508057
PMCPMC91575
OpenAlexW2156545952

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.