ArticleProceedings of the National Academy of Sciences of the United States of America1999
Slow transport of unpolymerized tubulin and polymerized neurofilament in the squid giant axon.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 77 citations in OpenAlex.
- Compartmentalized cytoplasmic tradewinds direct soluble proteins.Nature communications · 2026Article
- Microtubule retrograde flow retains neuronal polarization in a fluctuating state.Science advances · 2022Article
- The Giant Axon of the Squid: A Simple System for Axonal Transport Studies.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Mini-review: Microtubule sliding in neurons.Neuroscience letters · 2021Review
- Spatially regulated editing of genetic information within a neuron.Nucleic acids research · 2020Article
- Effect of Cyclic Stretch on Neuron Reorientation and Axon Outgrowth.Frontiers in bioengineering and biotechnology · 2020Article
- The nano-architecture of the axonal cytoskeleton.Nature reviews. Neuroscience · 2017Review
- Dendrite and Axon Specific Geometrical Transformation in Neurite Development.Frontiers in computational neuroscience · 2015Article
- Single-molecule tracking of tau reveals fast kiss-and-hop interaction with microtubules in living neurons.Molecular biology of the cell · 2014Article
- Seeing the unseen: the hidden world of slow axonal transport.The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry · 2014Review
- Article
- Modeling anterograde and retrograde transport of short mobile microtubules from the site of axonal branch formation.Journal of biological physics · 2014Article
- ERK2-mediated phosphorylation of Par3 regulates neuronal polarization.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Quantitative measurements and modeling of cargo-motor interactions during fast transport in the living axon.Physical biology · 2012Article
- Neurofilaments at a glance.Journal of cell science · 2012Review
- Article
- Kinesin-1/Hsc70-dependent mechanism of slow axonal transport and its relation to fast axonal transport.The EMBO journal · 2010Article
- No conventional function for the conventional kinesin?Traffic (Copenhagen, Denmark) · 2008Article
- DISC1 regulates neurotrophin-induced axon elongation via interaction with Grb2.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- DISC1 regulates the transport of the NUDEL/LIS1/14-3-3epsilon complex through kinesin-1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
A major issue in the slow transport of cytoskeletal proteins is the form in which they are transported. We have investigated the possibility that unpolymerized as well as polymerized cytoskeletal proteins can be actively transported in axons. We report the active transport of highly diffusible tubulin oligomers, as well as transport of the less diffusible neurofilament polymers. After injection into the squid giant axon, tubulin was transported in an anterograde direction at an average rate of 2.3 mm/day, whereas neurofilament was moved at 1.1 mm/day. Addition of the metabolic poisons cyanide or dinitrophenol reduced the active transport of both proteins to less than 10% of control values, whereas disruption of microtubules by treatment of the axon with cold in the presence of nocodazole reduced transport of both proteins to approximately 20% of control levels. Passive diffusion of these proteins occurred in parallel with transport. The diffusion coefficient of the moving tubulin in axoplasm was 8.6 micrometer(2)/s compared with only 0.43 micrometer(2)/s for neurofilament. These results suggest that the tubulin was transported in the unpolymerized state and that the neurofilament was transported in the polymerized state by an energy-dependent nocodazole/cold-sensitive transport mechanism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.