ArticleProceedings of the National Academy of Sciences of the United States of America1999
Atomic force microscopy captures length phenotypes in single proteins.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed.
- Membrane-Coated Microsphere Functionalization with Polypeptides.Biomacromolecules · 2026Article
- Lipid traffic: an enigmatic alliance between ORPs and a TMEM16-like protein at membrane contact sites.Biochemical Society transactions · 2026Review
- Tailoring the adhesion properties of thin polymeric films using additives: an AFM study.Nanoscale advances · 2026Article
- Ist2 promotes lipid transfer by Osh6 via its membrane tethering and lipid scramblase activities.Science advances · 2025Article
- Nanopore sensing of protein and peptide conformation for point-of-care applications.Nature communications · 2025Review
- Structural anisotropy results in mechano-directional transport of proteins across nuclear pores.Nature physics · 2024Article
- Lateral force separation of biopolymers using an atomic force microscope.Biomicrofluidics · 2023Article
- Evidence of Orientation-Dependent Early States of Prion Protein Misfolded Structures from Single Molecule Force Spectroscopy.Biology · 2022Article
- Mechanical Stability of Ribonuclease A Heavily Depends on the Redox Environment.The journal of physical chemistry. B · 2022Article
- Single-molecule displacement assay reveals strong binding of polyvalent dendrimer ligands to telomeric G-quadruplex.Analytical biochemistry · 2022Article
- Investigations on the substrate binding sites of hemolysin B, an ABC transporter, of a type 1 secretion system.Frontiers in microbiology · 2022Article
- Protein Unfolding: Denaturant vs. Force.Biomedicines · 2021Article
- Microbial production of megadalton titin yields fibers with advantageous mechanical properties.Nature communications · 2021Article
- BRCA1/BARD1 site-specific ubiquitylation of nucleosomal H2A is directed by BARD1.Nature structural & molecular biology · 2021Article
- Mechanical Stability of a Small, Highly-Luminescent Engineered Protein NanoLuc.International journal of molecular sciences · 2020Article
- Next Generation Methods for Single-Molecule Force Spectroscopy on Polyproteins and Receptor-Ligand Complexes.Frontiers in molecular biosciences · 2020Review
- Single-Molecule Force Spectroscopy on the N2A Element of Titin: Effects of Phosphorylation and CARP.Frontiers in physiology · 2020Article
- Nanomechanical properties of steric zipper globular structures.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Differential HDAC1/2 network analysis reveals a role for prefoldin/CCT in HDAC1/2 complex assembly.Scientific reports · 2018Article
- Unraveling the Mechanical Unfolding Pathways of a Multidomain Protein: Phosphoglycerate Kinase.Biophysical journal · 2018Article
11 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We use single-protein atomic force microscopy techniques to detect length phenotypes in an Ig module. To gain amino acid resolution, we amplify the mechanical features of a single module by engineering polyproteins composed of up to 12 identical repeats. We show that on mechanical unfolding, mutant polyproteins containing five extra glycine residues added to the folded core of the module extend 20 A per module farther than the wild-type polyproteins. By contrast, similar insertions near the N or C termini have no effect. Hence, our atomic force microscopy measurements readily discriminate the location of the insert and measure its size with a resolution similar to that of NMR and x-ray crystallography.
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