ArticleProceedings of the National Academy of Sciences of the United States of America1999
Vacuole acidification is required for trans-SNARE pairing, LMA1 release, and homotypic fusion.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- The Lack of a COPII Cargo Receptor Erv14 Impacts Physiological Functions of the Vacuole in Saccharomyces cerevisiae.Traffic (Copenhagen, Denmark) · 2026Article
- SNARE disassembly requires Sec18/NSF side loading.Nature structural & molecular biology · 2025Article
- SNARE disassembly requires Sec18/NSF side-loading.bioRxiv : the preprint server for biology · 2025Article
- Article
- Phosphatidylinositol 3,5-bisphosphate regulates CaTraffic (Copenhagen, Denmark) · 2020Article
- Copper blocks V-ATPase activity and SNARE complex formation to inhibit yeast vacuole fusion.Traffic (Copenhagen, Denmark) · 2019Article
- The inner workings of intracellular heterotypic and homotypic membrane fusion mechanisms.Journal of biosciences · 2019Article
- The V-ATPase V1 subunit A1 is required for rhodopsin anterograde trafficking in Drosophila.Molecular biology of the cell · 2018Article
- A mouse model of autism implicates endosome pH in the regulation of presynaptic calcium entry.Nature communications · 2018Article
- Requirement for Ergosterol in Berberine Tolerance Underlies Synergism of Fluconazole and Berberine against Fluconazole-ResistantFrontiers in cellular and infection microbiology · 2017Article
- The Coordinated Action of Calcineurin and Cathepsin D Protects Against α-Synuclein Toxicity.Frontiers in molecular neuroscience · 2017Article
- Target of rapamycin signaling mediates vacuolar fission caused by endoplasmic reticulum stress in Saccharomyces cerevisiae.Molecular biology of the cell · 2015Article
- Abscisic acid prevents the coalescence of protein storage vacuoles by upregulating expression of a tonoplast intrinsic protein gene in barley aleurone.Journal of experimental botany · 2015Article
- Vibrio effector protein VopQ inhibits fusion of V-ATPase-containing membranes.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Homotypic vacuole fusion in yeast requires organelle acidification and not the V-ATPase membrane domain.Developmental cell · 2013Article
- The V-ATPase membrane domain is a sensor of granular pH that controls the exocytotic machinery.The Journal of cell biology · 2013Article
- The vesicular ATPase: a missing link between acidification and exocytosis.The Journal of cell biology · 2013Article
- Identification of domains within the V-ATPase accessory subunit Ac45 involved in V-ATPase transport and Ca2+-dependent exocytosis.The Journal of biological chemistry · 2012Article
- Mitofusins and the mitochondrial permeability transition: the potential downside of mitochondrial fusion.American journal of physiology. Heart and circulatory physiology · 2012Review
- The endosomal Na(+)/H(+) exchanger contributes to multivesicular body formation by regulating the recruitment of ESCRT-0 Vps27p to the endosomal membrane.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vacuole fusion occurs in three stages: priming, in which Sec18p mediates Sec17p release, LMA1 (low M(r) activity 1) relocation, and cis-SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex disassembly; docking, mediated by Ypt7p and trans-SNARE association; and fusion of docked vacuoles. Ca(2+) and calmodulin regulate late stages of the reaction. We now show that the vacuole proton gradient, generated by the vacuolar proton ATPase, is needed for trans-SNARE complex formation during docking and hence for the subsequent LMA1 release. Though neither the vacuolar Pmc1p Ca(2+)-ATPase nor the Vcx1p Ca(2+)/H(+) exchanger are needed for the fusion reaction, they participate in Ca(2+) and Delta mu(H)(+) homeostasis. Fusion itself does not require the maintenance of trans-SNARE pairs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.