ArticleJournal of bacteriology1999
Analysis of F factor TraD membrane topology by use of gene fusions and trypsin-sensitive insertions.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Chimeric Coupling Proteins Mediate Transfer of Heterologous Type IV Effectors through the Escherichia coli pKM101-Encoded Conjugation Machine.Journal of bacteriology · 2016Article
- Rational engineering of a virulence gene from Mycobacterium tuberculosis facilitates proteomic analysis of a natural protein N-terminus.Scientific reports · 2016Article
- The All-Alpha Domains of Coupling Proteins from the Agrobacterium tumefaciens VirB/VirD4 and Enterococcus faecalis pCF10-Encoded Type IV Secretion Systems Confer Specificity to Binding of Cognate DNA Substrates.Journal of bacteriology · 2015Article
- Article
- Mobile genetic elements in the genus Bacteroides, and their mechanism(s) of dissemination.Mobile genetic elements · 2011Article
- Plasmid r1 conjugative DNA processing is regulated at the coupling protein interface.Journal of bacteriology · 2009Article
- Interaction of Bacteroides fragilis pLV22a relaxase and transfer DNA with Escherichia coli RP4-TraG coupling protein.Molecular microbiology · 2007Article
- In vivo oligomerization of the F conjugative coupling protein TraD.Journal of bacteriology · 2007Article
- General mutagenesis of F plasmid TraI reveals its role in conjugative regulation.Journal of bacteriology · 2006Article
- Characterization of a novel porin involved in systemic Yersinia enterocolitica infection.Infection and immunity · 2006Article
- Mutations in the C-terminal region of TraM provide evidence for in vivo TraM-TraD interactions during F-plasmid conjugation.Journal of bacteriology · 2005Article
- Biogenesis, architecture, and function of bacterial type IV secretion systems.Annual review of microbiology · 2005Review
- Type IV secretion: the Agrobacterium VirB/D4 and related conjugation systems.Biochimica et biophysica acta · 2004Review
- TraG-like proteins of type IV secretion systems: functional dissection of the multiple activities of TraG (RP4) and TrwB (R388).Journal of bacteriology · 2003Article
- TraG-like proteins of DNA transfer systems and of the Helicobacter pylori type IV secretion system: inner membrane gate for exported substrates?Journal of bacteriology · 2002Article
- Analysis of functional domains of the Enterococcus faecalis pheromone-induced surface protein aggregation substance.Journal of bacteriology · 2001Article
- Type IV secretion: intercellular transfer of macromolecules by systems ancestrally related to conjugation machines.Molecular microbiology · 2001Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
This report describes a procedure for characterizing membrane protein topology which combines the analysis of reporter protein hybrids and trypsin-sensitive 31-amino-acid insertions generated by using transposons ISphoA/in and ISlacZ/in. Studies of the F factor TraD protein imply that the protein takes on a structure with two membrane-spanning sequences and amino and carboxyl termini facing the cytoplasm. It was possible to assign the subcellular location of one region for which the behavior of fused reporter proteins was ambiguous, based on the trypsin cleavage behavior of a 31-residue insertion.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.