ArticleInfection and immunity1999
Genomic subtractive hybridization and selective capture of transcribed sequences identify a novel Salmonella typhimurium fimbrial operon and putative transcriptional regulator that are absent from the Salmonella typhi genome.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 49 citations in OpenAlex.
- Path-seq identifies an essential mycolate remodeling program for mycobacterial host adaptation.Molecular systems biology · 2019Article
- Formation of phenotypic lineages in Salmonella enterica by a pleiotropic fimbrial switch.PLoS genetics · 2018Article
- Article
- Molecular Characterization ofInternational journal of bacteriology · 2017Article
- Non-coding RNA regulation in pathogenic bacteria located inside eukaryotic cells.Frontiers in cellular and infection microbiology · 2014Review
- Identification of Salmonella enterica serovar Dublin-specific sequences by subtractive hybridization and analysis of their role in intestinal colonization and systemic translocation in cattle.Infection and immunity · 2008Article
- RosE represses Std fimbrial expression in Salmonella enterica serotype Typhimurium.Molecular microbiology · 2008Article
- Identification of Haemophilus ducreyi genes expressed during human infection.Microbiology (Reading, England) · 2008Article
- Evolution of the chaperone/usher assembly pathway: fimbrial classification goes Greek.Microbiology and molecular biology reviews : MMBR · 2007Review
- Transcriptome of Salmonella enterica serovar Typhi within macrophages revealed through the selective capture of transcribed sequences.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Identification of novel Salmonella enterica serovar Typhimurium DT104-specific prophage and nonprophage chromosomal sequences among serovar Typhimurium isolates by genomic subtractive hybridization.Applied and environmental microbiology · 2005Article
- In vivo expression of the mannose-resistant fimbriae of Photorhabdus temperata K122 during insect infection.Journal of bacteriology · 2004Article
- Novel virulence-associated type II secretion system unique to high-pathogenicity Yersinia enterocolitica.Infection and immunity · 2003Article
- Identification of pathogen-specific and conserved genes expressed in vivo by an avian pathogenic Escherichia coli strain.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- The evolving genome of Salmonella enterica serovar Pullorum.Journal of bacteriology · 2002Article
- Phenotypic and genomic analyses of the Mycobacterium avium complex reveal differences in gastrointestinal invasion and genomic composition.Infection and immunity · 2001Article
- Gene fragments distinguishing an epidemic-associated strain from a virulent prototype strain of Listeria monocytogenes belong to a distinct functional subset of genes and partially cross-hybridize with other Listeria species.Infection and immunity · 2001Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Salmonella typhi, the etiologic agent of typhoid fever, is adapted to the human host and unable to infect nonprimate species. The genetic basis for host specificity in S. typhi is unknown. The avirulence of S. typhi in animal hosts may result from a lack of genes present in the broad-host-range pathogen Salmonella typhimurium. Genomic subtractive hybridization was successfully employed to isolate S. typhimurium genomic sequences which are absent from the S. typhi genome. These genomic subtracted sequences mapped to 17 regions distributed throughout the S. typhimurium chromosome. A positive cDNA selection method was then used to identify subtracted sequences which were transcribed by S. typhimurium following macrophage phagocytosis. A novel putative transcriptional regulator of the LysR family was identified as transcribed by intramacrophage S. typhimurium. This putative transcriptional regulator was absent from the genomes of the human-adapted serovars S. typhi and Salmonella paratyphi A. Mutations within this gene did not alter the level of S. typhimurium survival within macrophages or virulence within mice. A subtracted genomic fragment derived from the ferrichrome operon also hybridized to the intramacrophage cDNA. Nucleotide sequence analysis of S. typhimurium and S. typhi chromosomal sequences flanking the ferrichrome operon identified a novel S. typhimurium fimbrial operon with a high level of similarity to sequences encoding Proteus mirabilis mannose-resistant fimbriae. The novel fimbrial operon was absent from the S. typhi genome. The absence of specific genes may have allowed S. typhi to evolve as a highly invasive, systemic human pathogen.
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Registered trials
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