ArticleInfection and immunity1999
Pseudomonas aeruginosa quorum-sensing signal molecule N-(3-oxododecanoyl)-L-homoserine lactone inhibits expression of P2Y receptors in cystic fibrosis tracheal gland cells.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 53 citations in OpenAlex.
- A bibliometric analysis on discovering anti-quorum sensing agents against clinically relevant pathogens: current status, development, and future directions.Frontiers in microbiology · 2023Pooled it
- EDTA and Taurolidine Affect Pseudomonas aeruginosa VirulenceMicrobiology spectrum · 2021Article
- Immunization with 3-oxododecanoyl-L-homoserine lactone-r-PcrV conjugate enhances survival of mice against lethal burn infections caused by Pseudomonas aeruginosa.Bosnian journal of basic medical sciences · 2015Article
- Role of PON2 in innate immune response in an acute infection model.Molecular genetics and metabolism · 2013Article
- Action of natural products on p2 receptors: a reinvented era for drug discovery.Molecules (Basel, Switzerland) · 2012Review
- Influence of Pseudomonas aeruginosa quorum sensing signal molecule N-(3-oxododecanoyl) homoserine lactone on mast cells.Medical microbiology and immunology · 2009Article
- Anti-inflammatory therapies for cystic fibrosis-related lung disease.Clinical reviews in allergy & immunology · 2008Review
- Pseudomonas aeruginosa autoinducer enters and functions in mammalian cells.Journal of bacteriology · 2004Article
- The Pseudomonas aeruginosa autoinducer N-3-oxododecanoyl homoserine lactone accelerates apoptosis in macrophages and neutrophils.Infection and immunity · 2003Article
- Extensive and specific responses of a eukaryote to bacterial quorum-sensing signals.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Identification of a quorum-sensing signal molecule in the facultative intracellular pathogen Brucella melitensis.Infection and immunity · 2002Article
- Pseudomonas aeruginosa quorum-sensing systems may control virulence factor expression in the lungs of patients with cystic fibrosis.Infection and immunity · 2002Article
- The Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxododecanoyl)homoserine lactone contributes to virulence and induces inflammation in vivo.Journal of bacteriology · 2002Article
- Haemodynamic effects of the bacterial quorum sensing signal molecule, N-(3-oxododecanoyl)-L-homoserine lactone, in conscious, normal and endotoxaemic rats.British journal of pharmacology · 2001Article
- Intercellular communication in Helicobacter pylori: luxS is essential for the production of an extracellular signaling molecule.Infection and immunity · 2000Article
- Quorum sensing and the population-dependent control of virulence.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2000Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ATP and UTP have been proposed for use as therapeutic treatment of the abnormal ion transport in the airway epithelium in cystic fibrosis (CF), the most characteristic feature of which is permanent infection by Pseudomonas aeruginosa. As for diverse gram-negative bacteria, this pathogenic bacterium accumulates diffusible N-acylhomoserine lactone (AHL) signal molecules, and when a threshold concentration is reached, virulence factor genes are activated. Human submucosal tracheal gland serous (HTGS) cells are believed to play a major role in the physiopathology of CF. Since ATP and UTP stimulate CF epithelial cells through P2Y receptors, we sought to determine whether CF HTGS cells are capable of responding to the AHLs N-butanoyl-L-homoserine lactone (BHL), N-hexanoyl-L-homoserine lactone (HHL), N-(3-oxododecanoyl)-L-homoserine lactone (OdDHL), and N-(3-oxohexanoyl)-L-homoserine lactone (OHHL), with special reference to P2Y receptors. All AHLs inhibited ATP- and UTP-induced secretion by CF HTGS cells. The 50% inhibitory concentrations were as high as 10 and 5 microM for BHL and HHL, respectively, but were only 0.3 and 0.4 pM for OdDHL and OHHL, respectively. Furthermore, all AHLs down-regulated the expression of the P2Y2 and P2Y4 receptors. Ibuprofen and nordihydroguaiaretic acid were able to prevent AHL inhibition of the responses to nucleotides, but neither dexamethasone nor indomethacin was able to do this. These data indicate that AHLs may alter responsiveness to ATP and UTP by CF HTGS cells and suggest that, in addition to ATP and/or UTP analogues, ibuprofen may be of use for a combinational pharmacological therapy for CF.
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