ArticleInfection and immunity1999
Cloning and sequencing of the genes downstream of the wbf gene cluster of Vibrio cholerae serogroup O139 and analysis of the junction genes in other serogroups.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Integrated analysis of O/K-antigen biosynthesis diversity and molecular serotyping scheme development of Vibrio alginolyticus.BMC microbiology · 2025Article
- VicPred: AFrontiers in microbiology · 2021Article
- High quality reference genomes for toxigenic and non-toxigenic Vibrio cholerae serogroup O139.Scientific reports · 2019Article
- Genomic comparison of serogroups O159 and O170 with other Vibrio cholerae serogroups.BMC genomics · 2019Article
- ISFrontiers in microbiology · 2019Article
- Genetic diversity of O-antigen biosynthesis regions in Vibrio cholerae.Applied and environmental microbiology · 2011Article
- Horizontal gene transfers with or without cell fusions in all categories of the living matter.Advances in experimental medicine and biology · 2011Review
- Serogroup conversion of Vibrio cholerae in aquatic reservoirs.PLoS pathogens · 2007Article
- The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: genes are embedded in the LPS region.BMC microbiology · 2007Article
- Multilocus sequence typing has better discriminatory ability for typing Vibrio cholerae than does pulsed-field gel electrophoresis and provides a measure of phylogenetic relatedness.Journal of clinical microbiology · 2003Article
- Comparative genomic analyses of the vibrio pathogenicity island and cholera toxin prophage regions in nonepidemic serogroup strains of Vibrio cholerae.Applied and environmental microbiology · 2003Article
- Emergence and evolution of Vibrio cholerae O139.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Genomic profiles of clinical and environmental isolates of Vibrio cholerae O1 in cholera-endemic areas of Bangladesh.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Evidence for the emergence of non-O1 and non-O139 Vibrio cholerae strains with pathogenic potential by exchange of O-antigen biosynthesis regions.Infection and immunity · 2002Article
- Characterization of VPI pathogenicity island and CTXphi prophage in environmental strains of Vibrio cholerae.Journal of bacteriology · 2001Article
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8 authors.
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Abstract
The DNA sequence of the O-antigen biosynthesis cluster (wbf) of a recently emergent pathogen, Vibrio cholerae serogroup O139, has been determined. Here we report the sequence of the genes downstream of the O139 wbfX gene and analysis of the genes flanking the wbf gene cluster in other serogroups. The gene downstream of wbfX, designated rjg (right junction gene), is predicted to be not required for O-antigen biosynthesis but appears to be a hot spot for DNA rearrangements. Several variants of the rjg gene (three different insertions and a deletion) have been found in other serogroups. DNA dot blot analysis of 106 V. cholerae strains showed the presence of the left and right junction genes, gmhD and rjg, respectively, in all strains. Further, these genes mapped to a single I-CeuI fragment in all 21 strains analyzed by pulsed-field gel electrophoresis, indicating a close linkage. The insertion sequence element IS1358, found in both O1 and O139 wb* regions, is present in 61% of the strains tested; interestingly, where present, it is predominantly linked to the wb* region. These results indicated a cassette-like organization of the wb* region, with the conserved genes (gmhD and rjg) flanking the divergent, serogroup-specific wb* genes and IS1358. A similar organization of the wb* region in other serogroups raises the possibility of the emergence of new pathogens by homologous recombination via the junction genes.
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