ReviewBiological chemistry
Chimeric restriction enzymes: what is next?
Review in Biological chemistry. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 85 citations in OpenAlex.
- Gene Editing and Human iPSCs in Cardiovascular and Metabolic Diseases.Advances in experimental medicine and biology · 2023Article
- Points of View on the Tools for Genome/Gene Editing.International journal of molecular sciences · 2021Review
- Targeted DNA transposition in vitro using a dCas9-transposase fusion protein.Nucleic acids research · 2019Article
- Expression of RecA and cell-penetrating peptide (CPP) fusion protein in bacteria and in mammalian cells.International journal of biochemistry and molecular biology · 2018Article
- Genome editing: a robust technology for human stem cells.Cellular and molecular life sciences : CMLS · 2017Review
- CRISPR-Cas Genome Surgery in Ophthalmology.Translational vision science & technology · 2017Review
- Origins of Programmable Nucleases for Genome Engineering.Journal of molecular biology · 2016Review
- Type II restriction endonucleases--a historical perspective and more.Nucleic acids research · 2014Review
- Natural zinc ribbon HNH endonucleases and engineered zinc finger nicking endonuclease.Nucleic acids research · 2013Article
- Repair at single targeted DNA double-strand breaks in pluripotent and differentiated human cells.PloS one · 2011Article
- Targeted genome engineering via zinc finger nucleases.Plant biotechnology reports · 2011Article
- Zinc-finger nuclease-induced gene repair with oligodeoxynucleotides: wanted and unwanted target locus modifications.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- Attenuation of zinc finger nuclease toxicity by small-molecule regulation of protein levels.PLoS genetics · 2009Article
- Custom-designed molecular scissors for site-specific manipulation of the plant and mammalian genomes.Methods in molecular biology (Clifton, N.J.) · 2009Article
- Binding of two zinc finger nuclease monomers to two specific sites is required for effective double-strand DNA cleavage.Biochemical and biophysical research communications · 2005Article
- Zinc finger nucleases: custom-designed molecular scissors for genome engineering of plant and mammalian cells.Nucleic acids research · 2005Review
- Common patterns in type II restriction enzyme binding sites.Nucleic acids research · 2005Article
- Therapeutic modulation of endogenous gene function by agents with designed DNA-sequence specificities.Nucleic acids research · 2003Review
- Diversity of type II restriction endonucleases that require two DNA recognition sites.Nucleic acids research · 2003Article
- Site-specific selfish genes as tools for the control and genetic engineering of natural populations.Proceedings. Biological sciences · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Chimeric restriction enzymes are a novel class of engineered nucleases in which the non-specific DNA cleavage domain of Fokl (a type IIS restriction endonuclease) is fused to other DNA-binding motifs. The latter include the three common eukaryotic DNA-binding motifs, namely the helix-turn-helix motif, the zinc finger motif and the basic helix-loop-helix protein containing a leucine zipper motif. Such chimeric nucleases have been shown to make specific cuts in vitro very close to the expected recognition sequences. The most important chimeric nucleases are those based on zinc finger DNA-binding proteins because of their modular structure. Recently, one such chimeric nuclease, Zif-QQR-F(N) was shown to find and cleave its target in vivo. This was tested by microinjection of DNA substrates and the enzyme into frog oocytes (Carroll et al., 1999). The injected enzyme made site-specific double-strand breaks in the targets even after assembly of the DNA into chromatin. In addition, this cleavage activated the target molecules for efficient homologous recombination. Since the recognition specificity of zinc fingers can be manipulated experimentally, chimeric nucleases could be engineered so as to target a specific site within a genome. The availability of such engineered chimeric restriction enzymes should make it feasible to do genome engineering, also commonly referred to as gene therapy.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.