ArticleThe Biochemical journal1999
Truncated isoforms inhibit [3H]prazosin binding and cellular trafficking of native human alpha1A-adrenoceptors.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 58 citations in OpenAlex.
- Catecholamines Activate a Tumour Progression Program in Differentiated Tumour Hepatocytes.Liver international : official journal of the International Association for the Study of the Liver · 2026Article
- Inhibitory effect of truncated isoforms on GPCR dimerization predicted by combinatorial computational strategy.Computational and structural biotechnology journal · 2024Article
- Histamine HBiomolecules · 2024Review
- Dynamic Dimerization of Chemokine Receptors and Potential Inhibitory Role of Their Truncated Isoforms Revealed through Combinatorial Prediction.International journal of molecular sciences · 2023Article
- Alternative Pre-mRNA Splicing of the Mu Opioid Receptor Gene,Biomolecules · 2021Review
- Dihydrofolate Reductase/Thymidylate Synthase Fine-Tunes the Folate Status and Controls Redox Homeostasis in Plants.The Plant cell · 2017Article
- Alternative Splicing of G Protein-Coupled Receptors: Relevance to Pain Management.Mayo Clinic proceedings · 2015Review
- The roles played by highly truncated splice variants of G protein-coupled receptors.Journal of molecular signaling · 2012Article
- G protein-coupled receptors: walking hand-in-hand, talking hand-in-hand?British journal of pharmacology · 2011Review
- GPR39: a Zn(2+)-activated G protein-coupled receptor that regulates pancreatic, gastrointestinal and neuronal functions.Cellular and molecular life sciences : CMLS · 2011Review
- Differential modulation of α-1 adrenoceptor subtypes by antidepressants in the rat brain.Journal of neural transmission (Vienna, Austria : 1996) · 2010Article
- A novel alternatively spliced isoform of the mu-opioid receptor: functional antagonism.Molecular pain · 2010Article
- Molecular characterization and analysis of a truncated serotonin receptor gene expressed in neural and reproductive tissues of abalone.Histochemistry and cell biology · 2009Article
- Combined use of alpha-adrenergic and muscarinic antagonists for the treatment of voiding dysfunction.The Journal of urology · 2005Review
- The specificity and molecular basis of alpha1-adrenoceptor and CXCR chemokine receptor dimerization.Journal of molecular neuroscience : MN · 2005Review
- Organization and expression of the SLC36 cluster of amino acid transporter genes.Mammalian genome : official journal of the International Mammalian Genome Society · 2004Article
- Alpha-1 adrenoceptors: evaluation of receptor subtype-binding kinetics in intact arterial tissues and comparison with membrane binding.British journal of pharmacology · 2004Article
- Genomic organization and characterization of splice variants of the human histamine H3 receptor.The Biochemical journal · 2001Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have identified from human liver eight alpha(1A)-adrenoceptor (alpha(1A)-AR) splice variants that were also expressed in human heart, prostate and hippocampus. Three of these alpha(1A)-AR isoforms (alpha(1A-1)-AR, alpha(1A-2a)-AR and alpha(1A-3a)-AR) gave rise to receptors with seven transmembrane domains (7TMalpha(1A)-AR). The other five (alpha(1A-2b)-AR, alpha(1A-2c)-AR, alpha(1A-3c)-AR, alpha(1A-5)-AR and alpha(1A-6)-AR) led to truncated receptors lacking transmembrane domain VII (6TMalpha(1A)-AR). The 7TMalpha(1A)-AR isoforms transiently expressed in COS-7 cells bound [(3)H]prazosin with high affinity (K(d) 0.2 nM) and mediated a noradrenaline (norepinephrine)-induced increase in cytoplasmic free Ca(2+) concentration, whereas the 6TMalpha(1A)-AR isoforms were incapable of ligand binding and signal transduction. Immunocytochemical studies with N-terminal epitope-tagged alpha(1A)-AR isoforms showed that the 7TMalpha(1A)-AR isoforms were present both at the cell surface and in intracellular compartments, whereas the 6TMalpha(1A)-AR isoforms were exclusively localized within the cell. Interestingly, in co-transfected cells, each truncated alpha(1A)-AR isoform inhibited [(3)H]prazosin binding and cell-surface trafficking of the co-expressed 'original' 7TMalpha(1A-1)-AR. However, there was no modification of either the [(3)H]prazosin-binding affinity or the pharmacological properties of alpha(1A-1)-AR. Immunoblotting experiments revealed that co-expression of the alpha(1A-1)-AR with 6TMalpha(1A)-AR isoforms did not impair alpha(1A-1)-AR expression. Therefore the expression in human tissues of many truncated isoforms constitutes a new regulation pathway of biological properties of alpha(1A)-AR.
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10493934PMC1220546W2034792871What OpenQuestion holds
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