ArticleThe Biochemical journal1999
HEK-293 cells possess a carbachol- and thapsigargin-sensitive intracellular Ca2+ store that is responsive to stop-flow medium changes and insensitive to caffeine and ryanodine.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 61 citations in OpenAlex.
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- A Superfolder Green Fluorescent Protein-Based Biosensor Allows Monitoring of Chloride in the Endoplasmic Reticulum.ACS sensors · 2022Article
- A BRET CaScience signaling · 2022Article
- Thapsigargin blocks electromagnetic field-elicited intracellular CaPhysiological reports · 2022Article
- Purinergic Receptors Crosstalk with CCR5 to Amplify CaCellular and molecular neurobiology · 2021Article
- Quantitative high-throughput assay to measure MC4R-induced intracellular calcium.Journal of molecular endocrinology · 2021Article
- MG53 suppresses interferon-β and inflammation via regulation of ryanodine receptor-mediated intracellular calcium signaling.Nature communications · 2020Article
- Australian ScorpionBiomolecules · 2020Article
- Sensing intracellular calcium ions using a manganese-based MRI contrast agent.Nature communications · 2019Article
- GPN does not release lysosomal CaJournal of cell science · 2019Article
- All three IPScience signaling · 2018Article
- Neuronal Calcium Recording with an Engineered TEV Protease.ACS chemical biology · 2018Article
- Generation and customization of biosynthetic excitable tissues for electrophysiological studies and cell-based therapies.Nature protocols · 2018Article
- Nanoluciferase Reporter Gene System Directed by Tandemly Repeated Pseudo-Palindromic NFAT-Response Elements Facilitates Analysis of Biological Endpoint Effects of Cellular CaInternational journal of molecular sciences · 2018Article
- HDAC Inhibition Improves the Sarcoendoplasmic Reticulum CaInternational journal of molecular sciences · 2018Article
- Endogenous signalling pathways and caged IPJournal of cell science · 2017Article
- Imaging Ca(2+) activity in mammalian cells and zebrafish with a novel red-emitting aequorin variant.Pflugers Archiv : European journal of physiology · 2015Article
- Quantifying SOCE fluorescence measurements in mammalian muscle fibres. The effects of ryanodine and osmotic shocks.Journal of muscle research and cell motility · 2013Article
- Involvement of presenilin holoprotein upregulation in calcium dyshomeostasis of Alzheimer's disease.Journal of cellular and molecular medicine · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Because HEK-293 cells are widely used for the functional expression of channels, exchangers and transporters involved in Ca(2+) homoeostasis, the properties of intracellular Ca(2+) stores and the methods used for measuring intracellular Ca(2+) release in HEK-293 cells were evaluated. Ca(2+) imaging was used to show caffeine-, carbachol- and thapsigargin-induced Ca(2+) release in HEK-293 cells transfected with ryanodine receptor (RyR) cDNA, but only carbachol- and thapsigargin-induced Ca(2+) release in untransfected HEK-293 cells. Intracellular Ca(2+) release in untransfected HEK-293 cells was also observed if medium changes were performed by aspirating and replacing fresh medium (stop-flow), but not if medium changes were performed by a continuous over-flow procedure. Stop-flow medium-change-induced Ca(2+) release in HEK-293 cells was independent of caffeine and ryanodine, demonstrating that it did not occur through RyR channels. Consistent with these observations was the observation that the level of expression of endogenous RyR proteins was below the limits of detection by Western blotting or [(3)H]ryanodine binding. Thus the level of endogenous expression of RyR is so low in HEK-293 cells as to provide a negligible background in relation to functional analysis of recombinant RyR molecules. These results are inconsistent with those of Querfurth et al. [Querfurth, Haughey, Greenway, Yacono, Golan and Geiger (1998) Biochem. J. 334, 79-86], who reported higher levels of endogenous RyR expression in untransfected HEK-293 cells.
Indexed as
Identifiers
10493909PMC1220521W1981389181What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.