ArticleBiochemical and biophysical research communications1999
The fluorescence emission of the apo-glucose oxidase from Aspergillus niger as probe to estimate glucose concentrations.
Article in Biochemical and biophysical research communications, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 76 citations in OpenAlex.
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- Engineered Glucose Oxidase-Carbon Nanotube Conjugates for Tissue-Translatable Glucose Nanosensors.Angewandte Chemie (International ed. in English) · 2024Article
- Article
- Biomimetic selenocystine based dynamic combinatorial chemistry for thiol-disulfide exchange.Nature communications · 2021Article
- One-pot HTST synthesis of responsive fluorescent ZnO@apo-enzyme composite microgels for intracellular glucometry.RSC advances · 2020Article
- Gel encapsulation of glucose nanosensors for prolonged in vivo lifetime.Journal of diabetes science and technology · 2013Article
- Fluorescence intensity- and lifetime-based glucose sensing using glucose/galactose-binding protein.Journal of diabetes science and technology · 2013Review
- Glucose response of near-infrared alginate-based microsphere sensors under dynamic reversible conditions.Diabetes technology & therapeutics · 2011Article
- Simultaneous determination of glucose and choline based on the intrinsic fluorescence of the enzymes.Journal of fluorescence · 2009Article
- Dissolved core alginate microspheres as "smart-tattoo" glucose sensors.Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · 2009Article
- Structure-based design of robust glucose biosensors using a Thermotoga maritima periplasmic glucose-binding protein.Protein science : a publication of the Protein Society · 2007Article
- Proteins from extremophiles as stable tools for advanced biotechnological applications of high social interest.Journal of the Royal Society, Interface · 2007Review
- Microscale enzymatic optical biosensors using mass transport limiting nanofilms. 1. Fabrication and characterization using glucose as a model analyte.Analytical chemistry · 2007Article
- Glucose sensors based on microcapsules containing an orange/red competitive binding resonance energy transfer assay.Diabetes technology & therapeutics · 2006Article
- Fluorescence glucose detection: advances toward the ideal in vivo biosensor.Journal of fluorescence · 2004Review
- Protein-based biosensors for diabetic patients.Journal of fluorescence · 2004Article
- Adenylation-dependent conformation and unfolding pathways of the NAD+-dependent DNA ligase from the thermophile Thermus scotoductus.Biophysical journal · 2004Article
- A novel fluorescence competitive assay for glucose determinations by using a thermostable glucokinase from the thermophilic microorganism Bacillus stearothermophilus.Analytical biochemistry · 2002Article
- Fluorescent probe for monosaccharides based on a functionalized boron-dipyrromethene with a boronic acid group.Tetrahedron letters · 2001Article
- Wavelength-ratiometric probes for saccharides based on donor-acceptor diphenylpolyenes.Journal of photochemistry and photobiology. A, Chemistry · 2001Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We developed a new method of glucose sensing using an inactive form of glucose oxidase from Aspergillus niger. Glucose oxidase was rendered inactive by removal of the FAD cofactor. The resulting apo-glucose oxidase still binds glucose as observed from a decrease in its intrinsic tryptophan fluorescence. 8-Anilino-1-naphthalenesulfonic acid (ANS) was found to bind spontaneously to apo-glucose oxidase as seen from an enhancement of the ANS fluorescence. The steady state intensity of the bound ANS decreased 25% upon binding of glucose, and the mean lifetime of the bound ANS decreased about 40%. These spectral changes occurred with a midpoint from 10 to 20 mM glucose, which is comparable to the K(D) of holo-glucose oxidase. These results suggest that apo-glucose oxidase can be used as a reversible nonconsuming sensor for glucose.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.