ArticlePathology oncology research : POR1999
Identification and characterization of different subpopulations in a human lung adenocarcinoma cell line (A549).
Article in Pathology oncology research : POR, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
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- Relevance of Host Cell Surface Glycan Structure for Cell Specificity of Influenza A Viruses.Viruses · 2023Article
- Production of a Granulysin-Based, Tn-Targeted Cytolytic Immunotoxin Using Pulsed Electric Field Technology.International journal of molecular sciences · 2020Article
- Single cell heterogeneity in influenza A virus gene expression shapes the innate antiviral response to infection.PLoS pathogens · 2020Article
- Process for an efficient lentiviral cell transduction.Biology methods & protocols · 2020Article
- Tumor suppressor properties of the small C-terminal domain phosphatases in non-small cell lung cancer.Bioscience reports · 2019Article
- Multilayered Cultures of NSCLC cells grown at the Air-Liquid Interface allow the efficacy testing of inhaled anti-cancer drugs.Scientific reports · 2018Article
- Article
- Breast cancer cells evade paclitaxel-induced cell death by developing resistance to dasatinib.Oncology letters · 2016Article
- Panels of tumor-derived RNA markers in peripheral blood of patients with non-small cell lung cancer: their dependence on age, gender and clinical stages.Oncotarget · 2016Article
- In vivo selection for spine-derived highly metastatic lung cancer cells is associated with increased migration, inflammation and decreased adhesion.Oncotarget · 2015Article
- NEU1 Sialidase Regulates Membrane-tethered Mucin (MUC1) Ectodomain Adhesiveness for Pseudomonas aeruginosa and Decoy Receptor Release.The Journal of biological chemistry · 2015Article
- MUC1 aptamer-based near-infrared fluorescence probes for tumor imaging.Molecular imaging and biology · 2015Article
- Differential Regulation of Gene Expression of Alveolar Epithelial Cell Markers in Human Lung Adenocarcinoma-Derived A549 Clones.Stem cells international · 2015Article
- Features of senescence and cell death induced by doxorubicin in A549 cells: organization and level of selected cytoskeletal proteins.Journal of cancer research and clinical oncology · 2010Article
- Deregulation of the CEACAM expression pattern causes undifferentiated cell growth in human lung adenocarcinoma cells.PloS one · 2010Article
- The effects of acrolein on peroxiredoxins, thioredoxins, and thioredoxin reductase in human bronchial epithelial cells.Toxicology · 2009Article
- Reductive activation of hexavalent chromium by human lung epithelial cells: generation of Cr(V) and Cr(V)-thiol species.Journal of inorganic biochemistry · 2008Article
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4 authors.
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Abstract
The morphology, cell growth, antigenic expression and tumorigenicity of cell subpopulations from the A549 lung adenocarcinoma isolated by Percoll gradient separation have been analysed. Four subpopulations were obtained (subpopulations A, B, C and D). Immunocytochemical analysis of several antigens was performed with monoclonal antibodies (MAbs): MUC1 mucin (C595, HMFG1 and HMFG2), MUC5B (PANH2); gp230 (PANH4); carbohydrate antigens including sialyl Lewis x (KM93), Tn antigen (83D4), Lewis y (C14); 5, 6, 8, 17 and 19 cytokeratins and p53. The cell population D tended to form cell aggregates that piled up on the monolayer similar to overgrowth cultures of the A549 parental cell line, whereas A, B and C cell subpopulations formed well spread monolayers. Both parental A549 and subpopulation D secreted abundant mucus. The topographic distribution and secretion production were correlated with tumorigenic assays since only subpopulation D grew in nude mice exhibiting reduced latency period; these characteristics correlated with the fast growth of the subpopulation D in vitro. Immunocytochemical analysis demonstrated that subpopulation D showed greater expression of MUC1 mucin and carbohydrate antigens such as Tn antigen, sialyl Lewis x and Lewis y and less expression of cytokeratins, p53, MUC5B and gp230; conversely, subpopulations A, B and C showed the opposite antigenic profile. Our results illustrate heterogeneity in the A549 cell line; subpopulations A, B and C retained characteristics of more differentiated adenocarcinoma while subpopulation D displayed features of a less differentiated tumor line.
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