ArticleGene therapy1999
Transfer of YACs up to 2.3 Mb intact into human cells with polyethylenimine.
Article in Gene therapy, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 84 citations in OpenAlex.
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- Exogenous artificial DNA forms chromatin structure with active transcription in yeast.Science China. Life sciences · 2022Article
- Gene expressing human artificial chromosome vectors: Advantages and challenges for gene therapy.Experimental cell research · 2020Review
- Efficient size-independent chromosome delivery from yeast to cultured cell lines.Nucleic acids research · 2017Article
- Programming biological operating systems: genome design, assembly and activation.Nature methods · 2014Article
- Assembly of eukaryotic algal chromosomes in yeast.Journal of biological engineering · 2013Article
- Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology.Nucleic acids research · 2010Article
- In vitro assessments of nanomaterial toxicity.Advanced drug delivery reviews · 2009Review
- Efficient siRNA delivery with non-viral polymeric vehicles.Pharmaceutical research · 2009Review
- Drug releasing systems in cardiovascular tissue engineering.Journal of cellular and molecular medicine · 2009Review
- One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- A novel human artificial chromosome gene expression system using herpes simplex virus type 1 vectors.EMBO reports · 2006Article
- Delivery systems for the direct application of siRNAs to induce RNA interference (RNAi) in vivo.Journal of biomedicine & biotechnology · 2006Article
- Polymers for DNA delivery.Molecules (Basel, Switzerland) · 2005Review
- Delivery of unmodified bioactive ribozymes by an RNA-stabilizing polyethylenimine (LMW-PEI) efficiently down-regulates gene expression.Gene therapy · 2002Article
- Transfection of large plasmids in primary human myoblasts.Gene therapy · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The transfer of large YAC DNA into human cells is a laborious procedure. High quality pulsed field gel purified DNA is required, which is easily sheared during manipulation before transfection or degraded in the endosome of the cell following transfection. NaCl and polyamines compact and prevent DNA from shearing, but may not consistently protect DNA after transfection. We investigated if other polycations such as poly-L-lysine (PLL) and polyethylenimine (PEI) could condense and protect large YAC DNA (up to 2.3 Mb) from being degraded after lipofection. DNA condensation was monitored by a gel retardation assay, and atomic force microscopy (AFM). DNA was retarded in the gel when complexed with high concentrations of PLL and PEI, indicating that DNA had condensed. However, AFM images of PLL-DNA complexes showed aggregates of DNA molecules resulting from incomplete condensation, whereas PEI-DNA complexes produced condensed particles approximately 30-60 nm. Exogenous PLL-DNA remained intact in 36% of positive clones after lipofection, whereas PEI-DNA was intact in 100% of positive clones. PEI is a better condensing reagent than PLL, protecting DNA from shearing and endosomal degradation, and assists in delivering YACs up to 2.3 Mb intact into human cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.