ArticleGene therapy1999
Poly-L-lysine improves gene transfer with adenovirus formulated in PLGA microspheres.
Article in Gene therapy, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 51 citations in OpenAlex.
- Construction and application of adenoviral vectors.Molecular therapy. Nucleic acids · 2023Review
- Development of a New Hyaluronic Acid Based Redox-Responsive Nanohydrogel for the Encapsulation of Oncolytic Viruses for Cancer Immunotherapy.Nanomaterials (Basel, Switzerland) · 2021Article
- Transient Chimeric Ad5/37 Fiber Enhances NK-92 Carrier Cell-Mediated Delivery of Oncolytic Adenovirus Type 5 to Tumor Cells.Molecular therapy. Methods & clinical development · 2020Article
- Extracellular Vesicles-Mimetic Encapsulation Improves Oncolytic Viro-Immunotherapy in Tumors With Low Coxsackie and Adenovirus Receptor.Frontiers in bioengineering and biotechnology · 2020Article
- A cost-effective method to enhance adenoviral transduction of primary murine osteoblasts and bone marrow stromal cells.Bone research · 2016Article
- Combination recombinant simian or chimpanzee adenoviral vectors for vaccine development.Vaccine · 2015Article
- Bioactive baculovirus nanohybrids for stent based rapid vascular re-endothelialization.Scientific reports · 2013Article
- Engineering biomaterial systems to enhance viral vector gene delivery.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Review
- Immune recognition of gene transfer vectors: focus on adenovirus as a paradigm.Frontiers in immunology · 2011Article
- Article
- The effects of Runx2 immobilization on poly (epsilon-caprolactone) on osteoblast differentiation of bone marrow stromal cells in vitro.Biomaterials · 2010Article
- Oncolytic viruses: do they have a role in anti-cancer therapy?Clinical medicine. Oncology · 2008Article
- Article
- Mechanistic differences in DNA nanoparticle formation in the presence of oligolysines and poly-L-lysine.Biomacromolecules · 2007Article
- Identification of sites in adenovirus hexon for foreign peptide incorporation.Journal of virology · 2005Article
- Structural and phylogenetic analysis of adenovirus hexons by use of high-resolution x-ray crystallographic, molecular modeling, and sequence-based methods.Journal of virology · 2003Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
In vivo gene transfer with recombinant adenovirus vectors can be hindered by the immunogenicity of the adenovirus capsid proteins. Previous work showed that formulation of the vector with biodegradable polymers such as poly-lactic-glycolic acid (PLGA), polyethylene glycol (PEG), or lipids, may shield the virus from inhibition by neutralizing antibodies. Formulation of adenovirus in PLGA microspheres also allowed for extended release in vitro. In experiments described here, we found that the surfactant used in the formation of the primary emulsion could significantly improve the overall yield of virus released. We also tested the effects of adding poly-L-lysine to adenovirus before encapsulation with PLGA. Our results show that although PLL did not effect the yield of virus encapsulated or released from the microspheres, it significantly improved the efficiency of gene transfer after release from the polymer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.