ArticleMolecular and cellular biology1999
Association of fission yeast Orp1 and Mcm6 proteins with chromosomal replication origins.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 104 citations in OpenAlex.
- Maf1 limits RNA polymerase III-directed transcription to preserve genomic integrity and extend lifespan.Cell cycle (Georgetown, Tex.) · 2021Article
- New Blood Biomarkers for the Diagnosis of AFP-Negative Hepatocellular Carcinoma.Frontiers in oncology · 2020Review
- Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing.Nature communications · 2016Article
- Orc1 Binding to Mitotic Chromosomes Precedes Spatial Patterning during G1 Phase and Assembly of the Origin Recognition Complex in Human Cells.The Journal of biological chemistry · 2015Article
- Mapping of Replication Origins in the X Inactivation Center of Vole Microtus levis Reveals Extended Replication Initiation Zone.PloS one · 2015Article
- Regulating DNA replication in eukarya.Cold Spring Harbor perspectives in biology · 2013Review
- The fission yeast minichromosome maintenance (MCM)-binding protein (MCM-BP), Mcb1, regulates MCM function during prereplicative complex formation in DNA replication.The Journal of biological chemistry · 2013Article
- Article
- Coordinated degradation of replisome components ensures genome stability upon replication stress in the absence of the replication fork protection complex.PLoS genetics · 2013Article
- DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.Molecular biology of the cell · 2012Article
- Mcm10 plays an essential role in origin DNA unwinding after loading of the CMG components.The EMBO journal · 2012Article
- Swi1 associates with chromatin through the DDT domain and recruits Swi3 to preserve genomic integrity.PloS one · 2012Article
- The origin recognition complex: a biochemical and structural view.Sub-cellular biochemistry · 2012Review
- Abundance of prereplicative complexes (Pre-RCs) facilitates recombinational repair under replication stress in fission yeast.The Journal of biological chemistry · 2011Article
- Mrc1 marks early-firing origins and coordinates timing and efficiency of initiation in fission yeast.Molecular and cellular biology · 2011Article
- Pre-replication complex proteins assemble at regions of low nucleosome occupancy within the Chinese hamster dihydrofolate reductase initiation zone.Nucleic acids research · 2011Article
- Auxin-inducible protein depletion system in fission yeast.BMC cell biology · 2011Article
- Article
- Chromatin immunoprecipitation of replication factors moving with the replication fork.Methods in molecular biology (Clifton, N.J.) · 2009Article
- Balance between distinct HP1 family proteins controls heterochromatin assembly in fission yeast.Molecular and cellular biology · 2008Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have previously shown that replication of fission yeast chromosomes is initiated in distinct regions. Analyses of autonomous replicating sequences have suggested that regions required for replication are very different from those in budding yeast. Here, we present evidence that fission yeast replication origins are specifically associated with proteins that participate in initiation of replication. Most Orp1p, a putative subunit of the fission yeast origin recognition complex (ORC), was found to be associated with chromatin-enriched insoluble components throughout the cell cycle. In contrast, the minichromosome maintenance (Mcm) proteins, SpMcm2p and SpMcm6p, encoded by the nda1(+)/cdc19(+) and mis5(+) genes, respectively, were associated with chromatin DNA only during the G(1) and S phases. Immunostaining of spread nuclei showed SpMcm6p to be localized at discrete foci on chromatin during the G(1) and S phases. A chromatin immunoprecipitation assay demonstrated that Orp1p was preferentially localized at the ars2004 and ars3002 origins of the chromosome throughout the cell cycle, while SpMcm6p was associated with these origins only in the G(1) and S phases. Both Orp1p and SpMcm6p were associated with a 1-kb region that contains elements required for autonomous replication of ars2004. The results suggest that the fission yeast ORC specifically interacts with chromosomal replication origins and that Mcm proteins are loaded onto the origins to play a role in initiation of replication.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.